[Histonet] Changing Alcohol Vendors

[Edward]

Validate all reagent changes. The core principle is that you have
introduced an uncharacterized variable to a validated system. Reagent
alcohol is typically ethanol denatured with methanol and/or isopropanol.
The exact ratio and type of denaturants can differ between vendors. These
components are not inert and can affect tissue hydration, dehydration
rates, and differentiation of eosin.

Edward Edmonds, HT, QIHC

On Wed, Jul 23, 2025, 11:44 AM Pairan, Kelly via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Good Morning,
> Our hospital is looking at switching our 100% alcohol and 95% alcohol to
> another vendor.  I have received mixed answers on whether or not we are
> going to need to a validation for this reagent swap when it comes to
> processing, staining and IHC.  What is the practice at your institution?
>
> Thanks,
> Kelly
>
> Kelly Pairan, HT(ASCP)CM, HQIPCM
> Technical Scientist Anatomic Pathology and Cytology
> OhioHealth Laboratory Systems
> Suite 210 North Medical Building
> 3535 Olentangy River Rd
> Columbus, OH 43214
>
> Email: kelly.pairan at ohiohealth.com<mailto:kelly.pairan at ohiohealth.com>
> Work: (614) 566-3575
> Cell: (614) 312-0104
>
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