From ssy at health.ucsd.edu  Thu Aug  7 07:28:51 2025
From: ssy at health.ucsd.edu (Sy, Sabrina)
Date: Thu, 7 Aug 2025 12:28:51 +0000
Subject: [Histonet] " yellow" xylene
Message-ID: <MW4PR19MB6652060DCF0D24D8D4A19155EA2CA@MW4PR19MB6652.namprd19.prod.outlook.com>

We have received a shipment of xylene of the same lot number.
We have noticed that some xylene bottles are " yellow". We use it both for tissue processing and staining.
Has anyone encountered any processing or staining issues with oxidized xylene?

Sabrina
San Diego, CA

From tbraud at redeemerhealth.org  Thu Aug  7 12:25:03 2025
From: tbraud at redeemerhealth.org (Terri  Braud)
Date: Thu, 7 Aug 2025 17:25:03 +0000
Subject: [Histonet] Yellow Xylene
Message-ID: <9cfbd7014d2b40aeb24231135436ee7f@redeemerhealth.org>

The cause of a yellow tint to newly purchased Xylene could be a plethora of things but to me it is a clear sign of either degradation or contamination and I would not use it.  Terri

Terri L. Braud, HT(ASCP)
HNL Laboratories for 
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3689
Fax: 215-938-2021
????????? Honesty
AccouNtability
??? AgiLity
??? CoLlaboration
? CoMpassion

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Subject: [EXTERNAL] Histonet Digest, Vol 261, Issue 1

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Today's Topics:

   1. " yellow" xylene (Sy, Sabrina)


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Message: 1
Date: Thu, 7 Aug 2025 12:28:51 +0000
From: "Sy, Sabrina" <ssy at health.ucsd.edu>
To: "histonet at lists.utsouthwestern.edu"
	<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] " yellow" xylene
Message-ID:
	<MW4PR19MB6652060DCF0D24D8D4A19155EA2CA at MW4PR19MB6652.namprd19.prod.outlook.com>
	
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We have received a shipment of xylene of the same lot number.
We have noticed that some xylene bottles are " yellow". We use it both for tissue processing and staining.
Has anyone encountered any processing or staining issues with oxidized xylene?

Sabrina
San Diego, CA


------------------------------

Subject: Digest Footer

_______________________________________________
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End of Histonet Digest, Vol 261, Issue 1
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From john at imebinc.com  Thu Aug  7 12:54:16 2025
From: john at imebinc.com (=?utf-8?Q?John_O=E2=80=99Brien?=)
Date: Thu, 7 Aug 2025 10:54:16 -0700
Subject: [Histonet] Histonet Digest, Vol 261, Issue 1
In-Reply-To: <mailman.1.1754586001.11394.histonet@lists.utsouthwestern.edu>
References: <mailman.1.1754586001.11394.histonet@lists.utsouthwestern.edu>
Message-ID: <F53D1F1B-816D-4C38-A805-0C75EB5BA4D3@imebinc.com>

Old xylene from bottom of container and cheap quality
May cause some processing issues such as artifacts,
Cheapest price not always best solution
John
IMEB


> On Aug 7, 2025, at 10:09?AM, histonet-request at lists.utsouthwestern.edu wrote:
> 
> ?Send Histonet mailing list submissions to
>    histonet at lists.utsouthwestern.edu
> 
> To subscribe or unsubscribe via the World Wide Web, visit
>    http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
>    histonet-request at lists.utsouthwestern.edu
> 
> You can reach the person managing the list at
>    histonet-owner at lists.utsouthwestern.edu
> 
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
> 
> 
> Today's Topics:
> 
>   1. " yellow" xylene (Sy, Sabrina)
> 
> 
> ----------------------------------------------------------------------
> 
> Message: 1
> Date: Thu, 7 Aug 2025 12:28:51 +0000
> From: "Sy, Sabrina" <ssy at health.ucsd.edu>
> To: "histonet at lists.utsouthwestern.edu"
>    <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] " yellow" xylene
> Message-ID:
>    <MW4PR19MB6652060DCF0D24D8D4A19155EA2CA at MW4PR19MB6652.namprd19.prod.outlook.com>
>    
> Content-Type: text/plain; charset="us-ascii"
> 
> We have received a shipment of xylene of the same lot number.
> We have noticed that some xylene bottles are " yellow". We use it both for tissue processing and staining.
> Has anyone encountered any processing or staining issues with oxidized xylene?
> 
> Sabrina
> San Diego, CA
> 
> 
> ------------------------------
> 
> Subject: Digest Footer
> 
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> ------------------------------
> 
> End of Histonet Digest, Vol 261, Issue 1
> ****************************************



From edward.edmonds at gmail.com  Sat Aug 16 01:56:30 2025
From: edward.edmonds at gmail.com (Edward)
Date: Sat, 16 Aug 2025 02:56:30 -0400
Subject: [Histonet] PGP 9.5
Message-ID: <CAJxa+a74b7akyrODNWVtMudP6kuFnnM6i7ajHx2Menh8P05uQg@mail.gmail.com>

I'm looking for someone who performs PGP 9.5 on skin biopsies in the US.

Would you be able to accept a biopsy that is shipped appropriately and
provide digital imaging without diagnostics?

Thank you,
Edward Edmonds, HT, QIHC

From edward.edmonds at gmail.com  Sat Aug 16 02:15:04 2025
From: edward.edmonds at gmail.com (Edward)
Date: Sat, 16 Aug 2025 03:15:04 -0400
Subject: [Histonet] Changing Alcohol Vendors
In-Reply-To: <DM3PR19MB8428509ED90FB11D2A9BF021F45FA@DM3PR19MB8428.namprd19.prod.outlook.com>
References: <DM3PR19MB8428509ED90FB11D2A9BF021F45FA@DM3PR19MB8428.namprd19.prod.outlook.com>
Message-ID: <CAJxa+a4pSjKHbtkBn63WCvr5rmSSqqs5-Q4Y6xJkH4Ac7xcpOA@mail.gmail.com>

Validate all reagent changes. The core principle is that you have
introduced an uncharacterized variable to a validated system. Reagent
alcohol is typically ethanol denatured with methanol and/or isopropanol.
The exact ratio and type of denaturants can differ between vendors. These
components are not inert and can affect tissue hydration, dehydration
rates, and differentiation of eosin.

Edward Edmonds, HT, QIHC

On Wed, Jul 23, 2025, 11:44?AM Pairan, Kelly via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Good Morning,
> Our hospital is looking at switching our 100% alcohol and 95% alcohol to
> another vendor.  I have received mixed answers on whether or not we are
> going to need to a validation for this reagent swap when it comes to
> processing, staining and IHC.  What is the practice at your institution?
>
> Thanks,
> Kelly
>
> Kelly Pairan, HT(ASCP)CM, HQIPCM
> Technical Scientist Anatomic Pathology and Cytology
> OhioHealth Laboratory Systems
> Suite 210 North Medical Building
> 3535 Olentangy River Rd
> Columbus, OH 43214
>
> Email: kelly.pairan at ohiohealth.com<mailto:kelly.pairan at ohiohealth.com>
> Work: (614) 566-3575
> Cell: (614) 312-0104
>
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>

From Amrit.Samra at hli.ubc.ca  Sat Aug 16 12:00:35 2025
From: Amrit.Samra at hli.ubc.ca (Amrit Samra)
Date: Sat, 16 Aug 2025 10:00:35 -0700
Subject: [Histonet] Histonet Digest, Vol 261, Issue 3
In-Reply-To: <F454F12002000048803483FE@mail.hli.ubc.ca>
References: <F454F12002000048803483FE@mail.hli.ubc.ca>
Message-ID: <68A0B9330200008D000CF28A@mail.hli.ubc.ca>

I will be away from the office from August 11 to August 15 inclusive.


From histo at pathlab.us  Tue Aug 19 12:31:00 2025
From: histo at pathlab.us (Histology)
Date: Tue, 19 Aug 2025 17:31:00 +0000
Subject: [Histonet] Nuclear fast red counterstain
Message-ID: <94015281c785445a89549531612cc2fc@pathlab.us>

Hi all,

Has anyone experienced commercially pre-made Nuclear Fast Red having reddish crystals in it?  We use this as a counterstain for Prussian Iron and Melanin stains and we are seeing little crystals on the slide.  We have tried filtering the solution but we are still seeing this problem.  Any help would be much appreciated.

Thanks as always Histonet!!

Mehndi Helgren
Lab Manager
757-664-7901
Dominion Pathology Labs.
733 Boush St. Suite 200
Norfolk, VA  23510


From jkiernan at uwo.ca  Tue Aug 19 14:32:29 2025
From: jkiernan at uwo.ca (John Kiernan)
Date: Tue, 19 Aug 2025 19:32:29 +0000
Subject: [Histonet] Nuclear fast red counterstain
In-Reply-To: <94015281c785445a89549531612cc2fc@pathlab.us>
References: <94015281c785445a89549531612cc2fc@pathlab.us>
Message-ID: <YT3PR01MB1082091292C9E6C777C08005BA530A@YT3PR01MB10820.CANPRD01.PROD.OUTLOOK.COM>

Insist on a solution made with nuclear fast red powder that has been certified by the Biological Stain Commission. Then you will know the dye really is CI  60760 and also that it works properly as a stain. See https://pubmed.ncbi.nlm.nih.gov/17510813/
[https://cdn.ncbi.nlm.nih.gov/pubmed/persistent/pubmed-meta-image-v2.jpg]<https://pubmed.ncbi.nlm.nih.gov/17510813/>
Certification procedures for nuclear fast red (Kernechtrot), CI 60760<https://pubmed.ncbi.nlm.nih.gov/17510813/>
Abstract Nuclear fast red (CI 60760), also known as Kernechtrot, is commonly used in conjunction with an excess of aluminum ions as a red nuclear counterstain following histochemical procedures that yield blue products. The dye has also been used as a histochemical and colorimetric reagent for calcium. Unsatisfactory samples of nuclear fast red are encountered occasionally, and confusion has ...
pubmed.ncbi.nlm.nih.gov
See also  https://biologicalstaincommission.org/new-dyes/, and search down the page for the item on nuclear fast red. This includes a warning about products that are sold as "certified" stains but have not been tested by the BSC.

Hoping this helps.  John Kiernan.
= = =
________________________________
From: Histology via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Tuesday, August 19, 2025 12:31 PM
To: Histonet <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Nuclear fast red counterstain

Hi all,

Has anyone experienced commercially pre-made Nuclear Fast Red having reddish crystals in it?  We use this as a counterstain for Prussian Iron and Melanin stains and we are seeing little crystals on the slide.  We have tried filtering the solution but we are still seeing this problem.  Any help would be much appreciated.

Thanks as always Histonet!!

Mehndi Helgren
Lab Manager
757-664-7901
Dominion Pathology Labs.
733 Boush St. Suite 200
Norfolk, VA  23510

_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

From john at imebinc.com  Wed Aug 20 12:51:55 2025
From: john at imebinc.com (=?utf-8?Q?John_O=E2=80=99Brien?=)
Date: Wed, 20 Aug 2025 10:51:55 -0700
Subject: [Histonet] Histonet Digest, Vol 261, Issue 5
In-Reply-To: <mailman.1.1755709201.10986.histonet@lists.utsouthwestern.edu>
References: <mailman.1.1755709201.10986.histonet@lists.utsouthwestern.edu>
Message-ID: <4FBD8C08-A84E-45BD-87C6-7E265A452F1E@imebinc.com>

Be sure stainer is not expired 
John IMEB Inc
Fresh stain less then 1 year old is best option we find

> On Aug 20, 2025, at 10:09?AM, histonet-request at lists.utsouthwestern.edu wrote:
> 
> ?Send Histonet mailing list submissions to
>    histonet at lists.utsouthwestern.edu
> 
> To subscribe or unsubscribe via the World Wide Web, visit
>    http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> or, via email, send a message with subject or body 'help' to
>    histonet-request at lists.utsouthwestern.edu
> 
> You can reach the person managing the list at
>    histonet-owner at lists.utsouthwestern.edu
> 
> When replying, please edit your Subject line so it is more specific
> than "Re: Contents of Histonet digest..."
> 
> 
> Today's Topics:
> 
>   1. Nuclear fast red counterstain (Histology)
>   2. Re: Nuclear fast red counterstain (John Kiernan)
> 
> 
> ----------------------------------------------------------------------
> 
> Message: 1
> Date: Tue, 19 Aug 2025 17:31:00 +0000
> From: Histology <histo at pathlab.us>
> To: Histonet <Histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Nuclear fast red counterstain
> Message-ID: <94015281c785445a89549531612cc2fc at pathlab.us>
> Content-Type: text/plain; charset="us-ascii"
> 
> Hi all,
> 
> Has anyone experienced commercially pre-made Nuclear Fast Red having reddish crystals in it?  We use this as a counterstain for Prussian Iron and Melanin stains and we are seeing little crystals on the slide.  We have tried filtering the solution but we are still seeing this problem.  Any help would be much appreciated.
> 
> Thanks as always Histonet!!
> 
> Mehndi Helgren
> Lab Manager
> 757-664-7901
> Dominion Pathology Labs.
> 733 Boush St. Suite 200
> Norfolk, VA  23510
> 
> 
> 
> ------------------------------
> 
> Message: 2
> Date: Tue, 19 Aug 2025 19:32:29 +0000
> From: John Kiernan <jkiernan at uwo.ca>
> To: Histology <histo at pathlab.us>, Amrit Samra via Histonet
>    <histonet at lists.utsouthwestern.edu>
> Subject: Re: [Histonet] Nuclear fast red counterstain
> Message-ID:
>    <YT3PR01MB1082091292C9E6C777C08005BA530A at YT3PR01MB10820.CANPRD01.PROD.OUTLOOK.COM>
>    
> Content-Type: text/plain; charset="iso-8859-1"
> 
> Insist on a solution made with nuclear fast red powder that has been certified by the Biological Stain Commission. Then you will know the dye really is CI  60760 and also that it works properly as a stain. See https://pubmed.ncbi.nlm.nih.gov/17510813/
> [https://cdn.ncbi.nlm.nih.gov/pubmed/persistent/pubmed-meta-image-v2.jpg]<https://pubmed.ncbi.nlm.nih.gov/17510813/>
> Certification procedures for nuclear fast red (Kernechtrot), CI 60760<https://pubmed.ncbi.nlm.nih.gov/17510813/>
> Abstract Nuclear fast red (CI 60760), also known as Kernechtrot, is commonly used in conjunction with an excess of aluminum ions as a red nuclear counterstain following histochemical procedures that yield blue products. The dye has also been used as a histochemical and colorimetric reagent for calcium. Unsatisfactory samples of nuclear fast red are encountered occasionally, and confusion has ...
> pubmed.ncbi.nlm.nih.gov
> See also  https://biologicalstaincommission.org/new-dyes/, and search down the page for the item on nuclear fast red. This includes a warning about products that are sold as "certified" stains but have not been tested by the BSC.
> 
> Hoping this helps.  John Kiernan.
> = = =
> ________________________________
> From: Histology via Histonet <histonet at lists.utsouthwestern.edu>
> Sent: Tuesday, August 19, 2025 12:31 PM
> To: Histonet <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Nuclear fast red counterstain
> 
> Hi all,
> 
> Has anyone experienced commercially pre-made Nuclear Fast Red having reddish crystals in it?  We use this as a counterstain for Prussian Iron and Melanin stains and we are seeing little crystals on the slide.  We have tried filtering the solution but we are still seeing this problem.  Any help would be much appreciated.
> 
> Thanks as always Histonet!!
> 
> Mehndi Helgren
> Lab Manager
> 757-664-7901
> Dominion Pathology Labs.
> 733 Boush St. Suite 200
> Norfolk, VA  23510
> 
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> 
> ------------------------------
> 
> Subject: Digest Footer
> 
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> 
> ------------------------------
> 
> End of Histonet Digest, Vol 261, Issue 5
> ****************************************



From amosbrooks at gmail.com  Thu Aug 21 22:17:38 2025
From: amosbrooks at gmail.com (Amos Brooks)
Date: Thu, 21 Aug 2025 23:17:38 -0400
Subject: [Histonet] Nuclear fast red counterstain
In-Reply-To: <mailman.1.1755709201.10986.histonet@lists.utsouthwestern.edu>
References: <mailman.1.1755709201.10986.histonet@lists.utsouthwestern.edu>
Message-ID: <CAC95ki_E4Re8FCzE4WSd1o4KavQQYoK+GyGkGaBVz7z_54HNAA@mail.gmail.com>

Hi,
     I make this up myself using BSC dye as John Kiernan recommends. I
still get the aluminum crystals as you describe with the
commercially purchased stain.
     Your best bet is to shake it up and re-dissolve the crystals. The
Potassium Aluminum sulfate is pretty saturated so it is likely to
precipitate especially if there is any evaporation of the solution. That
happens quickly if the solution gets old or is heated when used. It does
lead to a shorter shelf life than many other chemicals, but it still works
reasonably well.
     Just keep an eye on it. As it gets older it takes longer to work
(don't we all). Give it some more time or replace it (re-make it).

Happy staining,
Amos Brooks


Message: 1
Date: Tue, 19 Aug 2025 17:31:00 +0000
From: Histology <histo at pathlab.us>
To: Histonet <Histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Nuclear fast red counterstain
Message-ID: <94015281c785445a89549531612cc2fc at pathlab.us>
Content-Type: text/plain; charset="us-ascii"

Hi all,

Has anyone experienced commercially pre-made Nuclear Fast Red having
reddish crystals in it?  We use this as a counterstain for Prussian Iron
and Melanin stains and we are seeing little crystals on the slide.  We have
tried filtering the solution but we are still seeing this problem.  Any
help would be much appreciated.

Thanks as always Histonet!!

Mehndi Helgren
Lab Manager
757-664-7901
Dominion Pathology Labs.
733 Boush St. Suite 200
Norfolk, VA  23510