[Histonet] Cryostat knives vs disposable blades

[Colleen Forster]

LKatherione,

I have used the Sakura low profile Feather blades on mouse brains for years
with exceptional results. You do not need the steel or tungsten blades for
those.

I use those disopo blades for everything I cut in the cryostat, even
decalcified bones of small size.

Colleen Forster HT(AASCP)QIHC
UMN

On Thu, Nov 21, 2024 at 8:37 AM Davoli, Katherine A via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Hi Histonettles!
>
> I've got a bunch of projects coming up that require slicing frozen mouse
> brains.  Per my training, this should happen on knives rather than
> disposable steel blades ... we have the knives but they're in desperate
> need of sharpening, and it looks like not many places still do that
> service.  Here's my questions:
>
>
>   1.  CAN I use blades (Feather) instead of knives?  What will I be
> compromising to do it that way?
>
>   2.  If you use knives, what is a ballpark range of costs for sharpening
> them?
>
>   3.  If you use knives, who do you use to sharpen them?  Sturkey is who I
> used to use, but they don't offer this service anymore and have been
> referring people to a group in Atlanta instead.
>
> Any help you can provide would be greatly appreciated!
>
> Katherine Davoli, MDiv, HTL(ASCP)cm    (they/them/theirs)
> Lab Manager, Tissue Culture & Histology Cores, U. Pitt Dept of
> Ophthalmology
> 7.373 UPMC Mercy Pavilion        1622 Locust St., Pittsburgh PA 15219
> (412) 624-8508   this number cannot receive texts
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>


-- 
Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155
321 Church St. SE
Minneapolis, MN 55455
612-626-1930