[Histonet] vasculature staining in 3D

[Paula Keene Pierce]

Hi,
I have done retinal flat mounts to visualize vasculature with lectins and used chromagenic instead of fluoro.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

    On Wednesday, March 13, 2024 at 12:44:36 PM CDT, M.O. via Histonet <histonet at lists.utsouthwestern.edu> wrote:  
 
 Happy Wednesday!

I have an image in my head and I cannot find any image that matches what I
saw - cleared 3D tissue, maybe it was lungs or a heart with blue
vessels/veins stained.

I've done skeletal staining on mouse embryos with alcian blue and alizarin
red and was wondering if there is something similar for soft tissue and
vessels (not fluorescent). Is this something that exists?

I've seen vascular casting, but couldn't find images of cleared tissue with
stained vessels.

Thank you for any hints to my mystery!
-Merissa
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