[Histonet] Modified Davidson solution

[Colleen Forster]

I agree. Making some of these simple solutions that need to be FRESH is the
better way to go. As John said, there are ma ny books with these formulas
in them.

I have used Davidson's for different projects and I make mine in the lab
fresh each time tissue is harvested.

Colleen Forster HT(ASCP)QIHC

On Wed, Mar 6, 2024 at 5:26 AM Tony Henwood via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> I agree.
>
> A great book.
>
>
> Regards,
>
>
>
> Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired)
>
> Principal Scientist, the Children’s Hospital at Westmead (Retired)
>
> Adjunct Fellow, School of Medicine, University of Western Sydney.
>
> ________________________________
> From: John Kiernan via Histonet <histonet at lists.utsouthwestern.edu>
> Sent: 06 March 2024 18:32
> To: Histonet <histonet at lists.utsouthwestern.edu>; Naira Margaryan <
> nmargaryan88 at gmail.com>; Davoli, Katherine A <katherine.davoli at pitt.edu>
> Subject: Re: [Histonet] Modified Davidson solution
>
> The Davidson/Hartmann fixative is just another alcoholic formaldehyde
> mixture acidified with acetic acid. Like all others of that ilk it was
> intended for making up in the lab soon before using. Storage causes slow
> deterioration. The ethanol slowly gets esterified by the acetic acid,
> making ethyl acetate, which has no fixative properties. This change is
> evident from the fruity smell of the ester, which becomes evident after a
> week or two. All this has been in the textbooks for more than 50 years.
> Why doesn't every lab spend perhaps $200 on a couple of books?  For
> obvious reasons I recommend my 5th edition (ISBN 9781907904325; $82.50,from
> the publisher, which is $30 less than Amazon's price), but there are other
> histotechnology books that are very good.  A book may cost much less than a
> big bottle of a deteriorating mixture of questionable value, and you and
> your staff could learn a lot by reading.
> John Kiernan
> = = =
> ________________________________
> From: Davoli, Katherine A via Histonet <histonet at lists.utsouthwestern.edu>
> Sent: March 5, 2024 4:40 PM
> To: Histonet <histonet at lists.utsouthwestern.edu>; Naira Margaryan <
> nmargaryan88 at gmail.com>
> Subject: Re: [Histonet] Modified Davidson solution
>
> Hi Naira, I get Hartmann's from Electron Microscopy Sciences
> Cat# 64133-10 (for 1L, but they have larger sizes I believe)
>
>
> Katherine Davoli, HTL(ASCP)cm    (they/them/theirs)
>
> Lab Manager, Tissue Culture and Histology Core Facilities
>
> U. Pitt Dept of Ophthalmology
>
> 7.373 UPMC Mercy Pavilion
>
> 1622 Locust St., Pittsburgh PA 15219
>
> (412) 624-8508   this number cannot receive texts
>
> ________________________________
> From: Naira Margaryan via Histonet <histonet at lists.utsouthwestern.edu>
> Sent: Tuesday, March 5, 2024 1:27 PM
> To: Histonet <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Modified Davidson solution
>
> Hello!
>
> What is the best company to buy a ready to use Modified Davidson solution?
>
> Thanks in advance,
> Naira
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet<
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet>
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
> ________________________________
> From: Davoli, Katherine A via Histonet <histonet at lists.utsouthwestern.edu>
> Sent: March 5, 2024 4:40 PM
> To: Histonet <histonet at lists.utsouthwestern.edu>; Naira Margaryan <
> nmargaryan88 at gmail.com>
> Subject: Re: [Histonet] Modified Davidson solution
>
> Hi Naira, I get Hartmann's from Electron Microscopy Sciences
> Cat# 64133-10 (for 1L, but they have larger sizes I believe)
>
>
> Katherine Davoli, HTL(ASCP)cm    (they/them/theirs)
>
> Lab Manager, Tissue Culture and Histology Core Facilities
>
> U. Pitt Dept of Ophthalmology
>
> 7.373 UPMC Mercy Pavilion
>
> 1622 Locust St., Pittsburgh PA 15219
>
> (412) 624-8508   this number cannot receive texts
>
> ________________________________
> From: Naira Margaryan via Histonet <histonet at lists.utsouthwestern.edu>
> Sent: Tuesday, March 5, 2024 1:27 PM
> To: Histonet <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Modified Davidson solution
>
> Hello!
>
> What is the best company to buy a ready to use Modified Davidson solution?
>
> Thanks in advance,
> Naira
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet<
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet>
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>


-- 
Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155
321 Church St. SE
Minneapolis, MN 55455
612-626-1930