[Histonet] Modified Davidson solution

[John Kiernan]

The Davidson/Hartmann fixative is just another alcoholic formaldehyde mixture acidified with acetic acid. Like all others of that ilk it was intended for making up in the lab soon before using. Storage causes slow deterioration. The ethanol slowly gets esterified by the acetic acid, making ethyl acetate, which has no fixative properties. This change is evident from the fruity smell of the ester, which becomes evident after a week or two. All this has been in the textbooks for more than 50 years.
Why doesn't every lab spend perhaps $200 on a couple of books?  For obvious reasons I recommend my 5th edition (ISBN 9781907904325; $82.50,from the publisher, which is $30 less than Amazon's price), but there are other histotechnology books that are very good.  A book may cost much less than a big bottle of a deteriorating mixture of questionable value, and you and your staff could learn a lot by reading.
John Kiernan
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________________________________
From: Davoli, Katherine A via Histonet <histonet at lists.utsouthwestern.edu>
Sent: March 5, 2024 4:40 PM
To: Histonet <histonet at lists.utsouthwestern.edu>; Naira Margaryan <nmargaryan88 at gmail.com>
Subject: Re: [Histonet] Modified Davidson solution

Hi Naira, I get Hartmann's from Electron Microscopy Sciences
Cat# 64133-10 (for 1L, but they have larger sizes I believe)


Katherine Davoli, HTL(ASCP)cm    (they/them/theirs)

Lab Manager, Tissue Culture and Histology Core Facilities

U. Pitt Dept of Ophthalmology

7.373 UPMC Mercy Pavilion

1622 Locust St., Pittsburgh PA 15219

(412) 624-8508   this number cannot receive texts

________________________________
From: Naira Margaryan via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Tuesday, March 5, 2024 1:27 PM
To: Histonet <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Modified Davidson solution

Hello!

What is the best company to buy a ready to use Modified Davidson solution?

Thanks in advance,
Naira
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Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet<http://lists.utsouthwestern.edu/mailman/listinfo/histonet>
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

________________________________
From: Davoli, Katherine A via Histonet <histonet at lists.utsouthwestern.edu>
Sent: March 5, 2024 4:40 PM
To: Histonet <histonet at lists.utsouthwestern.edu>; Naira Margaryan <nmargaryan88 at gmail.com>
Subject: Re: [Histonet] Modified Davidson solution

Hi Naira, I get Hartmann's from Electron Microscopy Sciences
Cat# 64133-10 (for 1L, but they have larger sizes I believe)


Katherine Davoli, HTL(ASCP)cm    (they/them/theirs)

Lab Manager, Tissue Culture and Histology Core Facilities

U. Pitt Dept of Ophthalmology

7.373 UPMC Mercy Pavilion

1622 Locust St., Pittsburgh PA 15219

(412) 624-8508   this number cannot receive texts

________________________________
From: Naira Margaryan via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Tuesday, March 5, 2024 1:27 PM
To: Histonet <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Modified Davidson solution

Hello!

What is the best company to buy a ready to use Modified Davidson solution?

Thanks in advance,
Naira
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet<http://lists.utsouthwestern.edu/mailman/listinfo/histonet>
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet