[Histonet] Bone samples

[Simmons, Christopher]

Ion Exchange Decal containers will change your experience forever. 

-----Original Message-----
From: Gudrun Lang via Histonet <histonet at lists.utsouthwestern.edu> 
Sent: Friday, January 26, 2024 3:32 AM
To: 'Chakib Boussahmain' <chak_bou at yahoo.com>
Cc: histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Bone samples

Hi,
In my opinion the hardness of the decalcified blocks is often rather due to the paraffin-processing than the residual calcium. Especially when the tissue is decalcified really long. The hardness comes from the dehydration and "cooking" of collagen fibers. So additional decal will not help reducing the calcium, but helps to reintroduce water into the collagen-grid. And this is helpful for softening and cutting.
For myself, I often scratch the paraffin on the blocksurface away to face the bone directly to the water. Then I let them "swim" on my waterbath, until the surface is turned rather milky. After cooling again I cut in very very small steps to trim the surface. Sometimes it needs repeated swimming and cooling (and patience) to get a rather acceptable section. It is advantageous to pick them up on adhesive slides and let them dry in an 60°C oven to get rid of any residual water under the section.

Hope this helps
Gudrun

-----Ursprüngliche Nachricht-----
Von: Chakib Boussahmain via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Gesendet: Mittwoch, 24. Jänner 2024 23:34
An: histonet at lists.utsouthwestern.edu
Betreff: [Histonet] Bone samples


Hi guys,

 I hope this messagefinds you well. I am currently working on a study involving bone samples thathave been treated with slow decal and embedded in paraffin. I am facingchallenges in obtaining nice sections, and I was wondering if you could providesome guidance or recommendations.

Thank you in advance for your help!
Chakib
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