[Histonet] Bone samples

[Cooper, Brian]

Embed the bones diagonally in your molds if you're able (size depending) as this will allow for the greatest amount of paraffin support.  Trim them very slowly, and if need be, place the blocks into the same "slow decal" solution for additional amount of time sufficient to enable better sectioning.  Start checking in half hour or 45 minute intervals; rinse the blocks well in running water and attempt to section.  If they're not ready, back into decal solution they go.  

I feel that very wet ice helps to facilitate sectioning better than ice that is drier and fresh out of the freezer.  Just be sure to blot the face of the block with gauze before attempting to cut.  

There's my two cents!    

Thanks, 

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor 
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles 
4650 Sunset Blvd MS#43- Los Angeles, CA 90027 
Ph: 323.361.3357
bcooper at chla.usc.edu 

-----Original Message-----
From: Chakib Boussahmain via Histonet <histonet at lists.utsouthwestern.edu> 
Sent: Wednesday, January 24, 2024 2:34 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Bone samples (EXTERNAL EMAIL)

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Hi guys,

 I hope this messagefinds you well. I am currently working on a study involving bone samples thathave been treated with slow decal and embedded in paraffin. I am facingchallenges in obtaining nice sections, and I was wondering if you could providesome guidance or recommendations.

Thank you in advance for your help!
Chakib
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