[Histonet] tissue cassettes

[Mac Donald, Jennifer]

We embed from cold and this happens if the tissue is not brought up to the temperature and the wax is melted.

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From: Carl Hobbs via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Friday, February 9, 2024 11:12 AM
To: histonet <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] tissue cassettes

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I'm interested but don't understand the variation and it's + effect I take my cassettes out of the processor and immediately place into the molten wax bath of the embedder ( if I'm embedding immediately; if not I let the cassettes/tissues therein go cold until a later embedding) When embedding immediately, after 30 mins, I remove tissue from cassette into a heated metal mold filled with molten wax All done quickly, to keep all wax molten If my people take too long, the wax around the tissue sets so when the tissue is placed into the molten wax, in mold, they sometimes get that effect of set wax-interface artefact....the section immediately pulls apart from the surrounding wax, on the waterbath This can cause the section to fold Do enlighten me

Thanks

Carl
Never too old to learn!


Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson SPaRC
Guys Campus, London Bridge
Kings College London
London
SE1 1UL

020 7848 6810
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