[Histonet] Modified Davidson

[John Kiernan]

The "Davidson's" name is, as Tony says, applied to various mixtures. In these, formalin is acidified with acetic acid in solutions that also contain some alcohol (typically about 33% v/v) but probably not enough to contribute to fixation. The mixture quoted from Latendresse et al. 2002 has only 15% alcohol - about the same as in a good dry sherry.  (My gastric mucosa is not yet fixed.)

The Histonet archives must surely still contain Dr Bob Richmond's contributions about "Davidson's fixative" in the 1990s or 2000s.

Enough from me for now!
John Kiernan
London, Canada
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________________________________
From: Tony Henwood via Histonet <histonet at lists.utsouthwestern.edu>
Sent: February 8, 2024 5:31 PM
To: Naira Margaryan <nmargaryan88 at gmail.com>; Histonet <histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Modified Davidson

There are several formulations (some are probably typos), but this seems to be one commonly cited.

Modified Davidson's Fixative:

(Latendresse, J. R., Warbrittion, A. R., Jonassen, H., & Creasy, D. M. (2002). Fixation of testes and eyes using a modified Davidson's fluid: comparison with Bouin's fluid and conventional Davidson's fluid. Toxicologic pathology, 30(4), 524-533.)

37–40% formaldehyde            30ml
Absolute ethanol         15ml
Glacial acetic acid        5ml
Distilled Water             50ml

Regards,

Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired)
Principal Scientist, the Children’s Hospital at Westmead (Retired)
Adjunct Fellow, School of Medicine, University of Western Sydney.

________________________________
From: Naira Margaryan via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Friday, February 9, 2024 8:25:47 AM
To: Histonet <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Modified Davidson

Hello,

Could you please share your best recipe for the Modified Davidson?

Thanks in advance,
Naira
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