[Histonet] Antibody dilution help

[Paula Sicurello]

Charles,
The easiet way to remember it is this:  you want a 1:100 dilution - so you want 1 part antibody in 100 parts of diluent.  I was taught that meant add 1 part antibody to 100 parts of diluent.  However, there are some who add 1 part anitbody to 99 parts diluent.Thus you end up with 1 part antibody in 100 parts total.
Some folks read 1:100 as 1 part in 100, some folks read it as add 1 part to 99 to make 100 parts.
confused yet?
 I found this


Let's see what others have to say.
Sincerely,
Paula Sicurello 

    On Friday, August 30, 2024 at 07:57:36 AM PDT, Charles Riley via Histonet <histonet at lists.utsouthwestern.edu> wrote:  
 
 Hello everyone,

Not sure if it's just burnout or Covid brain but I am having issues with
remembering the right way to dilute my antibodies, and all the calculators
online seem to give different responses.

I have 100 microliters of 1mg/mL antibody and I want to make a 1:100
dilution.

How much diluent do I add to do this?  And if anyone has any calculators
that they like using that would be awesome too
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet