[Histonet] Oven Temps and baking times used for IHC's

Fri Aug 9 07:51:34 CDT 2024

I tap and pat my slides dry on a Kimwipe.  Place them n my Gemini
Autostainer which has an onboard heater.  I heat the slides for 20 minutes
and then they get deparaffinized.  Then I proceed with HIER if needed and
then on to the Intellipath.  Never had an issue,
NEW EMAIL:Karen.Schieberl at UCSF.edu
Karen Schieberl HT ASCP

Lead Histology Technician

St. Mary's Medical Center

450 Stanyan St.

San Francisco, Ca. 94117

415-750-5751

karen.schieberl@ <karen.schieberl at dignityhealth.org>commonspirit.org

NEW EMAIL:Karen.Schieberl at UCSF.edu

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On Fri, Aug 9, 2024 at 12:44 AM Gudrun Lang via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> USE CAUTION - EXTERNAL EMAIL
>
> ......................................................................
> Hallo!
> Sections are dried at room-temperature vertically to get rid of the water
> beneath the sections. For automated staining they aren't baked any more.
> For semi-manual staining with HIER in the pressure cooker they were baked
> at 60°C for 30-60 min.
>
> Dry temperatures above 70°C influence the results of IHC.
> Williams JH, Mepham BL, Wright DH (1997) Tissuepreparation for
> immunocytochemistry J Clin Pathol 50:422–428
>
> Gudrun Lang
> Kepleruniversitätsklinik Linz, Austria
>
> -----Ursprüngliche Nachricht-----
> Von: Eileen Akemi Allison via Histonet [mailto:
> histonet at lists.utsouthwestern.edu]
> Gesendet: Donnerstag, 8. August 2024 18:23
> An: Histonet
> Betreff: [Histonet] Oven Temps and baking times used for IHC's
>
> Good morning Histo Peeps!
>
> I am taking a poll on what various labs are using for temperature and
> times for IHC’s slides which are baked in the oven.  It has been my
> experience while working at Biocare Medical, we would 1st wick excess water
> off the slides, then tap the rack of slides on paper; then subsequently
> place the rack of slides in a 36 degree oven for 30 minutes, then up the
> temperature to 60-62 degrees for an additional time of 30-60 minutes.
> Sorry, my brain cells can’t remember which length of time in 60 degrees, I
> left Biocare Medical in 2006.  My ex, David Tacha, Ph.D, HT/HTL gave
> several workshops on this subject internationally.
>
> The UCSF Surgical Pathology lab 1st wicks excess water off, then bakes
> their IHC slides at 62 degrees for 2 hours.  I have consulted w/ some labs
> who bake their IHC slides at 76 degrees for 20 minutes.  It has been my
> experience that higher than 62 degree temps will make the Ab lose their
> antigenicity.  CK Ab’s love a higher temp but most other Ab’s do not.
>
> Any thoughts, information, and papers on this subject would be greatly
> appreciated.  Thank you and have a wonderful upcoming weekend!
>
> Best regards,
> Akemi
>
> Akemi Allison-Tacha BS, HT/HTL (ASCP)
> Pathology Laboratory Manager at Golden State Dermatology
> 370 N Wiget Lane, Suite 250, Walnut Creek. CA 94598
> Cell: (408) 335-9994
> Email: aallison at gsdermca.com <mailto:aallison at gsdermca.com>
> Website:
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