[Histonet] Re IHC help

[Robyn L Vazquez]

Hello,
Another part of the process is to wick the tissue and place in front of a fan for about 10mins before placing in the 60 degrees oven. Just a suggestion.

Robyn

-----Original Message-----
From: Catherine Anne Heyward via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Thursday, August 8, 2024 5:10 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Re IHC help

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______________________________________________________________________
Hi,

If HIER at 90 degrees C is causing tissue lifting, you could try a lower temperature for longer. I was recommended 60 degrees overnight for bone/cartilage and have had far fewer problems with this gentler treatment. Perhaps this would suit the skin samples too.

Best wishes,

Cath


Catherine Heyward Ph.D

Senior technician - Oral Research Laboratory Local senior health and safety representative - Faculty for Dentistry

Tel: +47 9009 3113

Oral Research Laboratory
Institute for Clinical Dentistry
Postbox 1109 Blindern
0317 Oslo

Geitmyrsveien 69/71
0455 Oslo


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