[Histonet] [EXTERNAL] Epredia xylene substitute

[Gudrun Lang]

Hi Ann,
we have used ShellSol for years in our VIPs until our supplier stopped it. That's also a kind of naphta with aliphatic hydrogencarbons.
After that wie changed to the Sakura-substitute with the same protocol. For standard processing we had/have two stations with 60 min each at 40 °C. For fat processing the time is doubled.
That works for our specimens well enough.
Gudrun

-----Ursprüngliche Nachricht-----
Von: Ann Specian via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Gesendet: Freitag, 26. April 2024 02:07
An: Will Cavett
Cc: Histonet
Betreff: Re: [Histonet] [EXTERNAL] Epredia xylene substitute

Hi Will, great to hear from you!Yes those are all the reasons why we are considering it, but we would like to start validating tissue and rather than reinvent the wheel, We are looking for Processing protocols that have already been tried and true. If you have any,  if you could share them, it would be greatly appreciated.


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On Thursday, April 25, 2024, 6:39 PM, Will Cavett <wcavett at umc.edu> wrote:

I hope you are doing well, Ann? We considered Epredia's because it seems to offer a safer and more convenient option than xylene. Also, the evaporation rate of Epredia's Xylene Substitute is comparable to that of xylene, ensuring consistent performance in tissue processing .And, unlike xylene, which has a strong odor, Epredia's Xylene Substitute is virtually odorless. And lastly, Its airborne exposure limit is three times safer than xylene. Hope this helps.

Will Cavett, II

-----Original Message-----
From: Ann Specian via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Wednesday, April 24, 2024 2:00 PM
To: histonet at lists.utsouthwestern.edu
Subject: [EXTERNAL][Histonet] Epredia xylene substitute

We are thinking of changing from xylene to this substitute. Does anybody have any Processing protocols using Epredia Xylene substitute that they could share?


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