[Histonet] IF staining questions (Charles Riley)

[Katy Milne]

If you want a stronger signal I would probably use the secondary antibody vs using a directly conjugated CD11c.

You could use a directly conjugated one as you would for flow cytometry but it won't give as much amplification as it would with a secondary step built in.

Katy

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Today's Topics:

   1. IF staining questions (Charles Riley)


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Message: 1
Date: Thu, 19 Oct 2023 09:22:56 -0400
From: Charles Riley <criley at udel.edu>
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] IF staining questions
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        <CAHMJNCjWAbcXgcHuzs657idSMpLhQEY=JKs6oZooeLwQDT3E1w at mail.gmail.com>
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Why would one decide to use a primary antibody along with a secondary antibody rather than a primary antibody conjugated with the secondary?

Example.   I have a researcher who wants to do CD11C staining with
Alexafluor488

Is it better to buy and use a primary antibody CD11C conjugated with
Alexafluor488  or to do the CD11C primary and a Rabbit anti-rat (H_L) IgG antibody secondary?


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