[Histonet] IF staining questions

[Charles Riley]

Why would one decide to use a primary antibody along with a secondary
antibody rather than a primary antibody conjugated with the secondary?

Example.   I have a researcher who wants to do CD11C staining with
Alexafluor488

Is it better to buy and use a primary antibody CD11C conjugated with
Alexafluor488  or to do the CD11C primary and a Rabbit anti-rat (H_L) IgG
antibody secondary?