[Histonet] Fixing and processing mouse eyeballs

[Paula Keene Pierce]

Hi,
I have done 10s of thousands of mice eyes, as well as other species, processed to paraffin in my career with no retinal detachment and enhanced IHC and ISH, but using my own method.

Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

    On Thursday, October 12, 2023 at 01:43:39 PM CDT, Li, Nathan via Histonet <histonet at lists.utsouthwestern.edu> wrote:  
 
 The J.Pang paper article John Kieran referenced might not be do-able in your lab without glyoxal but they have another excellent article here with more routine techniques:  https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8585658/

Nathan Li, BS. HTL(ASCP)CM QIHC
Histology Manager
Tufts University
Tufts Comparative Medicine Services
Animal Histology Core
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-----Original Message-----
From: Charles Riley <criley at udel.edu> 
Sent: Wednesday, October 11, 2023 5:00 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Fixing and processing mouse eyeballs

Does anyone have any helpful tips or protocols for fixing and processing schedules for adult mouse eyes?

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