[Histonet] Faded H&E tissue section

[Amos Brooks]

Hi,
    Don't decolonize. Soak the coverslip off and let it sit in xylene to
make absolutely sure all the mounting media is off then simply re-stain it.
If there is any mounting media left on it will interfere with the staining.
Unless there's something wrong with either the section or the reagents, it
should work just fine.

Amos Brooks

Message: 6
Date: Thu, 9 Nov 2023 22:02:46 +0530
From: "jayalakshmy p.s" <psjayalakshmy at gmail.com>
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Faded H&E tissue section
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Hello,
I would like to know how to effectively restain a faded H&E tissue section.
The color becomes dull when re stained. Somebody please advise.
Prof. Jayalakshmy. P S