[Histonet] Sections sticking to tweezers

[Kurth, Virginia L]

You may try to turn your water bath down a few degrees and see if this helps and what is the melting point of the paraffin you are using?  Most likely a paraffin/heat situation.
 If I  run into this issue, a quick fix is I rinse my forceps into the  ice water, wipe, and the sections will not stick.

Good Luck.

Ginny Kurth
UWHEALTH

-----Original Message-----
From: Ken M via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Saturday, May 6, 2023 10:27 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Sections sticking to tweezers

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Can anyone tell me why my sections are sticking to my tweezers and turning into one long string of snot when I try to separate them? I have a good pair of curved stainless tweezers and I am trying both the front and the back and tapping sharply while allowing the tweezers to open at the separation point. I can separate one or two, then it sticks and pulls up 7 or 8 sections into one long slimy string. My water bath is set at 38.5 and I am sectioning at 4-5 m. Even when I go to 6 it still does it. Any ideas?

Ken
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