[Histonet] Sudanblack B on FFPET

[Gudrun Lang]

Many thanks to all for your helpful hints.

Kind regards
Gudrun Lang

-----Ursprüngliche Nachricht-----
Von: Tony Henwood via Histonet [mailto:histonet at lists.utsouthwestern.edu] 
Gesendet: Dienstag, 28. März 2023 21:13
An: Bob Richmond; Histonet at lists.utsouthwestern.edu
Betreff: Re: [Histonet] Sudanblack B on FFPET

I would also let the saturated solution stand for a few days. Like Oil Red
O, it probably needs time to “mature”. I would also use a frozen section of
skin as a control.

Regards,

Tony Henwood
Sydney, Australia

From: Bob Richmond via Histonet<mailto:histonet at lists.utsouthwestern.edu>
Sent: Wednesday, 29 March 2023 4:51 AM
To:
Histonet at lists.utsouthwestern.edu<mailto:histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Sudanblack B on FFPET

>
> Gudrun Lang in Austria asks:
>

>>Has anyone experience with Sudanblack B on paraffin slides for staining
[lipofuscin]? A doctor wants the demonstration of the lipoid content of
foamy cells or granulocytes in lung. I've found protocols that have
incubation-times from 10 minutes to over-night. - I've made a saturated
Sudan black B-solution in 70% ethanol and tried it with10 min on liver
without real success.<<

The main thing you need to do is demonstrate that it isn't hemosiderin with
an iron stain (Perls prussian blue reaction), and perhaps also that it
isn't acid-fast. Lipofuscin can be identified an H & E staining, except for
these considerations.

Bob Richmond
Maryville, Tennessee
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