[Histonet] IHC gunk on slides
Eddie Martin
edmartin26 at gmail.com
Fri Mar 24 09:48:18 CDT 2023
Karen,
Regardless of the instrument you’re using, if related to a reagent, it may
be that your detection chromogen is breaking down and you need to report
it, or you have a material that is prepared onsite, and used onboard the
instrument, and unknowingly contaminated.
it may help if you can provide an image. It can be done with a mobile phone
up to the eyepiece if necessary.
Best wishes,
Eddie Martin
The National Institutes of Health
301-594-2054
On Thu, Mar 23, 2023 at 1:00 PM <histonet-request at lists.utsouthwestern.edu>
wrote:
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> Today's Topics:
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> 1. IHC gunk (Karen Heckford CA-San Francisco)
> 2. Re: IHC gunk (Willis, Donna G)
> 3. Re: IHC gunk (Thomas Podawiltz)
> 4. Re: IHC gunk (Mac Donald, Jennifer)
> 5. Re: Histonet Digest, Vol 232, Issue 7 (Eddie Martin)
>
>
>
> ---------- Forwarded message ----------
> From: Karen Heckford CA-San Francisco <karen.heckford at commonspirit.org>
> To: Histonet <histonet at lists.utsouthwestern.edu>
> Cc:
> Bcc:
> Date: Wed, 22 Mar 2023 10:45:25 -0700
> Subject: [Histonet] IHC gunk
> Good Morning,
> I have recently developed a problem with contamination of some kind on my
> IHC slides.
> The contamination is black clumps and lays on top of the tissue. I have
> been told it is bacteria but the Pathologist and I kind of doubt that. I
> cannot get a good picture of it to show. It may not be on all the slides
> on the same run or even the same antibody slide.
>
> I have decontaminated the whole system. I have put fresh reagents on the
> instrument. This stuff looks like it would wash off at the end of the run
> since it is sitting right on top of the tissue. I do not see this stuff
> elsewhere on the slide, only the tissue. Does not matter if the tissue
> was cut fresh or not.
>
> I have tried everything I can think of to get rid of it and still have this
> issue. I use DiH20 from the hospital system. Not sure if this may be the
> problem. I can have Engineering test the DiH20.
>
> Any help would be greatly appreciated.
>
> Thanks,
>
> Karen Heckford HT ASCP CE
>
> Lead Histology Technician
>
> St. Mary's Medical Center
>
> 450 Stanyan St.
>
> San Francisco, Ca. 94117
>
> 415-750-5751
>
> karen.heckford@ <karen.heckford at dignityhealth.org>commonspirit.org
>
>
>
>
> Caution: This email message, including all content and attachments, is
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> intended recipient or an agent responsible for delivering it to the
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> notify us immediately by reply email. Thank you
>
> Caution: This email is both proprietary and confidential, and not intended
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> you.
>
>
>
>
> ---------- Forwarded message ----------
> From: "Willis, Donna G" <Donna.Willis at bswhealth.org>
> To: Karen Heckford CA-San Francisco <karen.heckford at commonspirit.org>,
> Histonet <histonet at lists.utsouthwestern.edu>
> Cc:
> Bcc:
> Date: Wed, 22 Mar 2023 17:57:09 +0000
> Subject: Re: [Histonet] IHC gunk
> What instrumentation are you using. Have you talked to your vendor?
>
> Donna Willis
> Anatomic Pathology Manager
> Baylor Scott&White Health
> Baylor University Medical Center
> 3500 Gaston Ave|Dallas, Texas 75246
> 214-820-2465 office|214-725-6184 mobile
>
>
>
> -----Original Message-----
> From: Karen Heckford CA-San Francisco via Histonet <
> histonet at lists.utsouthwestern.edu>
> Sent: Wednesday, March 22, 2023 12:45 PM
> To: Histonet <histonet at lists.utsouthwestern.edu>
> Subject: {EXTERNAL} [Histonet] IHC gunk
>
>
> CAUTION: This email originated outside of BSWH. The actual sender is (
> histonet-bounces at lists.utsouthwestern.edu) which may be different from
> the display address in the From: field. Avoid action unless you know the
> content is safe. Report suspicious emails using the PhishAlarm button
> located in your Outlook ribbon.
>
> Good Morning,
> I have recently developed a problem with contamination of some kind on my
> IHC slides.
> The contamination is black clumps and lays on top of the tissue. I have
> been told it is bacteria but the Pathologist and I kind of doubt that. I
> cannot get a good picture of it to show. It may not be on all the slides
> on the same run or even the same antibody slide.
>
> I have decontaminated the whole system. I have put fresh reagents on the
> instrument. This stuff looks like it would wash off at the end of the run
> since it is sitting right on top of the tissue. I do not see this stuff
> elsewhere on the slide, only the tissue. Does not matter if the tissue
> was cut fresh or not.
>
> I have tried everything I can think of to get rid of it and still have
> this issue. I use DiH20 from the hospital system. Not sure if this may be
> the problem. I can have Engineering test the DiH20.
>
> Any help would be greatly appreciated.
>
> Thanks,
>
> Karen Heckford HT ASCP CE
>
> Lead Histology Technician
>
> St. Mary's Medical Center
>
> 450 Stanyan St.
>
> San Francisco, Ca. 94117
>
> 415-750-5751
>
> karen.heckford@ <karen.heckford at dignityhealth.org>commonspirit.org
>
>
>
>
> Caution: This email message, including all content and attachments, is
> CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The
> information contained in this email message is intended only for the use of
> the recipient(s) named above. If the reader of this message is not the
> intended recipient or an agent responsible for delivering it to the
> intended recipient, you have received this document in error. Any further
> review, dissemination, distribution, or copying of this message is strictly
> prohibited. If you have received this communication in error, please
> notify us immediately by reply email. Thank you
>
> Caution: This email is both proprietary and confidential, and not intended
> for transmission to (or receipt by) any unauthorized person(s). If you
> believe that you have received this email in error, do not read any
> attachments. Instead, kindly reply to the sender stating that you have
> received the message in error. Then destroy it and any attachments. Thank
> you.
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
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>
> **********************************************************************
> The information contained in this e-mail may be privileged, confidential,
> and/or protected from disclosure. If you are the intended recipient,
> further disclosures are prohibited without proper authorization. If you are
> not the intended recipient (or have received this e-mail in error) please
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> copying, disclosure or distribution of the material in this e-mail is
> strictly prohibited and no waiver of any attorney-client, work product, or
> other privilege is intended. No binding agreement on behalf of Baylor Scott
> & White Health, or any affiliated entity, is permitted by e-mail without
> express written confirmation by a duly authorized representative of Baylor
> Scott & White Health.
>
>
>
> ---------- Forwarded message ----------
> From: Thomas Podawiltz <tpodawiltz at yahoo.com>
> To: "Willis, Donna G" <Donna.Willis at bswhealth.org>, Karen Heckford CA-San
> Francisco <karen.heckford at commonspirit.org>, Histonet <
> histonet at lists.utsouthwestern.edu>
> Cc:
> Bcc:
> Date: Wed, 22 Mar 2023 18:02:32 +0000 (UTC)
> Subject: Re: [Histonet] IHC gunk
> Is it on all slides or just certain antibodies?
>
>
> Sent from Yahoo Mail for iPhone
>
>
> On Wednesday, March 22, 2023, 1:57 PM, Willis, Donna G via Histonet <
> histonet at lists.utsouthwestern.edu> wrote:
>
> What instrumentation are you using. Have you talked to your vendor?
>
> Donna Willis
> Anatomic Pathology Manager
> Baylor Scott&White Health
> Baylor University Medical Center
> 3500 Gaston Ave|Dallas, Texas 75246
> 214-820-2465 office|214-725-6184 mobile
>
>
>
> -----Original Message-----
> From: Karen Heckford CA-San Francisco via Histonet <
> histonet at lists.utsouthwestern.edu>
> Sent: Wednesday, March 22, 2023 12:45 PM
> To: Histonet <histonet at lists.utsouthwestern.edu>
> Subject: {EXTERNAL} [Histonet] IHC gunk
>
>
> CAUTION: This email originated outside of BSWH. The actual sender is (
> histonet-bounces at lists.utsouthwestern.edu) which may be different from
> the display address in the From: field. Avoid action unless you know the
> content is safe. Report suspicious emails using the PhishAlarm button
> located in your Outlook ribbon.
>
> Good Morning,
> I have recently developed a problem with contamination of some kind on my
> IHC slides.
> The contamination is black clumps and lays on top of the tissue. I have
> been told it is bacteria but the Pathologist and I kind of doubt that. I
> cannot get a good picture of it to show. It may not be on all the slides
> on the same run or even the same antibody slide.
>
> I have decontaminated the whole system. I have put fresh reagents on the
> instrument. This stuff looks like it would wash off at the end of the run
> since it is sitting right on top of the tissue. I do not see this stuff
> elsewhere on the slide, only the tissue. Does not matter if the tissue
> was cut fresh or not.
>
> I have tried everything I can think of to get rid of it and still have
> this issue. I use DiH20 from the hospital system. Not sure if this may be
> the problem. I can have Engineering test the DiH20.
>
> Any help would be greatly appreciated.
>
> Thanks,
>
> Karen Heckford HT ASCP CE
>
> Lead Histology Technician
>
> St. Mary's Medical Center
>
> 450 Stanyan St.
>
> San Francisco, Ca. 94117
>
> 415-750-5751
>
> karen.heckford@ <karen.heckford at dignityhealth.org>commonspirit.org
>
>
>
>
> Caution: This email message, including all content and attachments, is
> CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The
> information contained in this email message is intended only for the use of
> the recipient(s) named above. If the reader of this message is not the
> intended recipient or an agent responsible for delivering it to the
> intended recipient, you have received this document in error. Any further
> review, dissemination, distribution, or copying of this message is strictly
> prohibited. If you have received this communication in error, please
> notify us immediately by reply email. Thank you
>
> Caution: This email is both proprietary and confidential, and not intended
> for transmission to (or receipt by) any unauthorized person(s). If you
> believe that you have received this email in error, do not read any
> attachments. Instead, kindly reply to the sender stating that you have
> received the message in error. Then destroy it and any attachments. Thank
> you.
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
>
> https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!JA_k2roV-A!FwKdOPOzj_UZTnMVxT9qbcCJsUPm3F4e3bDrGK4DAjDyfuxQPVCf46_156AghOFHjVm2UeSVHSgv4hZS98GaWcNtyAQS7nLY$
>
> **********************************************************************
> The information contained in this e-mail may be privileged, confidential,
> and/or protected from disclosure. If you are the intended recipient,
> further disclosures are prohibited without proper authorization. If you are
> not the intended recipient (or have received this e-mail in error) please
> notify the sender immediately and destroy this e-mail. Any unauthorized
> copying, disclosure or distribution of the material in this e-mail is
> strictly prohibited and no waiver of any attorney-client, work product, or
> other privilege is intended. No binding agreement on behalf of Baylor Scott
> & White Health, or any affiliated entity, is permitted by e-mail without
> express written confirmation by a duly authorized representative of Baylor
> Scott & White Health.
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
> http://lists.utsouthwestern.edu/mailman/listinfo/histonet
>
>
>
>
>
>
>
> ---------- Forwarded message ----------
> From: "Mac Donald, Jennifer" <jmacdonald at mtsac.edu>
> To: Karen Heckford CA-San Francisco <karen.heckford at commonspirit.org>,
> Histonet <histonet at lists.utsouthwestern.edu>
> Cc:
> Bcc:
> Date: Wed, 22 Mar 2023 18:15:21 +0000
> Subject: Re: [Histonet] IHC gunk
> Is it possible that it is hematoxylin precipitate?
>
> -----Original Message-----
> From: Karen Heckford CA-San Francisco via Histonet <
> histonet at lists.utsouthwestern.edu>
> Sent: Wednesday, March 22, 2023 10:45 AM
> To: Histonet <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] IHC gunk
>
> EXTERNAL SENDER - Exercise caution with requests, links, and attachments.
>
> Good Morning,
> I have recently developed a problem with contamination of some kind on my
> IHC slides.
> The contamination is black clumps and lays on top of the tissue. I have
> been told it is bacteria but the Pathologist and I kind of doubt that. I
> cannot get a good picture of it to show. It may not be on all the slides
> on the same run or even the same antibody slide.
>
> I have decontaminated the whole system. I have put fresh reagents on the
> instrument. This stuff looks like it would wash off at the end of the run
> since it is sitting right on top of the tissue. I do not see this stuff
> elsewhere on the slide, only the tissue. Does not matter if the tissue
> was cut fresh or not.
>
> I have tried everything I can think of to get rid of it and still have
> this issue. I use DiH20 from the hospital system. Not sure if this may be
> the problem. I can have Engineering test the DiH20.
>
> Any help would be greatly appreciated.
>
> Thanks,
>
> Karen Heckford HT ASCP CE
>
> Lead Histology Technician
>
> St. Mary's Medical Center
>
> 450 Stanyan St.
>
> San Francisco, Ca. 94117
>
> 415-750-5751
>
> karen.heckford@ <karen.heckford at dignityhealth.org>commonspirit.org
>
>
>
>
> Caution: This email message, including all content and attachments, is
> CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED. The
> information contained in this email message is intended only for the use of
> the recipient(s) named above. If the reader of this message is not the
> intended recipient or an agent responsible for delivering it to the
> intended recipient, you have received this document in error. Any further
> review, dissemination, distribution, or copying of this message is strictly
> prohibited. If you have received this communication in error, please
> notify us immediately by reply email. Thank you
>
> Caution: This email is both proprietary and confidential, and not intended
> for transmission to (or receipt by) any unauthorized person(s). If you
> believe that you have received this email in error, do not read any
> attachments. Instead, kindly reply to the sender stating that you have
> received the message in error. Then destroy it and any attachments. Thank
> you.
> _______________________________________________
> Histonet mailing list
> Histonet at lists.utsouthwestern.edu
>
> https://nam12.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C01%7Cjmacdonald%40mtsac.edu%7C27946f7c47f74e58436a08db2afd6592%7Ccc4d4bf20a9e4240aedea7d1d688f935%7C0%7C0%7C638151040067352929%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=coq7k8kIhG03oke%2B477suyaDCCby1zEujMseWZ4lQSw%3D&reserved=0
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>
>
>
>
> ---------- Forwarded message ----------
> From: Eddie Martin <edmartin26 at gmail.com>
> To: histonet at lists.utsouthwestern.edu, "kdean70 at hotmail.com" <
> kdean70 at hotmail.com>
> Cc:
> Bcc:
> Date: Wed, 22 Mar 2023 15:47:53 -0400
> Subject: Re: [Histonet] Histonet Digest, Vol 232, Issue 7
> Hi KDean70 at hotmail.com,
>
> What likely is occurring but you didn’t mention in the thread is that your
> friend is using alkaline phosphatase based detection, which, by itself can
> react with kidney tissue. This likely explains why your friend is getting
> strong kidney staining and weak melan-a staining.
>
> This also tells me your friend didn’t optimize the antibody correctly. I’m
> not sure why your friend is choosing to use a lyophilized antibody for
> Melan-A as so many other commercially available options are available
> capable of refrigerated storage and good up to 3 years. CellMarque makes an
> Melan-A in both realty to use and/or a liquid concentrate with a 3-year
> expiry date.
>
> Best wishes,
> Eddie Martin, HTL,QIHC
> The National Institutes of Health
> Department of Laboratory Medicine
> Bone Marrow Service
> Eddie.martin at nih.gov
> (301)-594-2054
>
>
>
> On Wed, Mar 15, 2023 at 1:00 PM <histonet-request at lists.utsouthwestern.edu
> >
> wrote:
>
> > Send Histonet mailing list submissions to
> > histonet at lists.utsouthwestern.edu
> >
> > To subscribe or unsubscribe via the World Wide Web, visit
> > http://lists.utsouthwestern.edu/mailman/listinfo/histonet
> > or, via email, send a message with subject or body 'help' to
> > histonet-request at lists.utsouthwestern.edu
> >
> > You can reach the person managing the list at
> > histonet-owner at lists.utsouthwestern.edu
> >
> > When replying, please edit your Subject line so it is more specific
> > than "Re: Contents of Histonet digest..."
> > Today's Topics:
> >
> > 1. Problems with Melan A staining (Ken M)
> >
> >
> >
> > ---------- Forwarded message ----------
> > From: Ken M <kdean70 at hotmail.com>
> > To: "histonet at lists.utsouthwestern.edu" <
> histonet at lists.utsouthwestern.edu
> > >
> > Cc:
> > Bcc:
> > Date: Wed, 15 Mar 2023 16:51:03 +0000
> > Subject: [Histonet] Problems with Melan A staining
> > One of my histotech friends is having issues with Melan A staining. Using
> > MA5-27949 Melan A antibody from Thermofisher, the negative Kidney tissue
> > control was stained strongly positive while the positive melanoma tissue
> > control was stained weakly positive. He couldn’t figure out why after
> > several tries to reduce background. Can someone give me some suggestions?
> > Thank you very much!
> >
> >
> > Ken
> >
> >
> > _______________________________________________
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