[Histonet] Tissue Processor Schedule Validations

[Paula Sicurello]

The one thing that bothers me about the preloaded programs is the paraffin temperature is set at 65 degrees C.  That's close to 10 degrees higher than the melting point of our paraffin.
Does anyone know why the temp. is so high?  To me, a temp that high will cause problems with IHC and small biopsies.  
Has anyone changed it?  Or do folks go along with it just because it's already there? 
I inherited that temp, to change it now would be pain since it is the one we use the most.  If I validated our current Peloris 3, I would have set the wax temperatures at 60 degrees.
Sincerely,
Paula Sicurello 

    On Thursday, March 23, 2023 at 11:54:44 AM PDT, Cooper, Brian via Histonet <histonet at lists.utsouthwestern.edu> wrote:  
 
 Good afternoon Histonet,

We're going to be validating a new tissue processor (Peloris 3) in the coming months, and I'm curious how people have validated small tissue processing protocols (GI bx's, liver/renal needle cores).  Larger tissues are much easier to do because we can readily gross duplicate sections. Obviously we can't adopt this approach for smaller samples because they're entirely submitted.  I have a game plan in mind, but would love some additional input! How'd you do it?

Thanks,

Brian D. Cooper, HT (ASCP)CMQIHCCM| Histology Supervisor
Department of Pathology and Laboratory Medicine
Children's Hospital Los Angeles
4650 Sunset Blvd MS#43- Los Angeles, CA 90027
Ph: 323.361.3357
bcooper at chla.usc.edu<mailto:bcooper at chla.usc.edu>

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