[Histonet] IHC gunk

[Willis, Donna G]

What instrumentation are you using.  Have you talked to your vendor?

Donna Willis
Anatomic Pathology Manager
Baylor Scott&White Health
Baylor University Medical Center
3500 Gaston Ave|Dallas, Texas 75246
214-820-2465 office|214-725-6184 mobile



-----Original Message-----
From: Karen Heckford CA-San Francisco via Histonet <histonet at lists.utsouthwestern.edu> 
Sent: Wednesday, March 22, 2023 12:45 PM
To: Histonet <histonet at lists.utsouthwestern.edu>
Subject: {EXTERNAL} [Histonet] IHC gunk


CAUTION:  This email originated outside of BSWH. The actual sender is  (histonet-bounces at lists.utsouthwestern.edu) which may be different from the display address in the From: field. Avoid action unless you know the content is safe. Report suspicious emails using the PhishAlarm button located in your Outlook ribbon.

Good Morning,
I have recently developed a problem with contamination of some kind on my IHC slides.
The contamination is black clumps and lays on top of the tissue.  I have
been told it is bacteria but the Pathologist and I kind of doubt that.   I
cannot get a good picture of it to show.  It may not be on all the slides on the same run or even the same antibody slide.

I have decontaminated the whole system.  I have put fresh reagents on the
instrument.   This stuff looks like it would wash off at the end of the run
since it is sitting right on top of the tissue.  I do not see this stuff
elsewhere on the slide, only the tissue.   Does not matter if the tissue
was cut fresh or not.

I have tried everything I can think of to get rid of it and still have this issue.  I use DiH20 from the hospital system.  Not sure if this may be the problem.  I can have Engineering test the DiH20.

Any help would be greatly appreciated.

Thanks,

Karen Heckford HT ASCP CE

Lead Histology Technician

St. Mary's Medical Center

450 Stanyan St.

San Francisco, Ca. 94117

415-750-5751

karen.heckford@ <karen.heckford at dignityhealth.org>commonspirit.org




Caution:  This email message, including all content and attachments, is CONFIDENTIAL and may be of a nature that is LEGALLY PRIVILEGED.  The information contained in this email message is intended only for the use of the recipient(s) named above. If the reader of this message is not the intended recipient or an agent responsible for delivering it to the intended recipient, you have received this document in error.  Any further review, dissemination, distribution, or copying of this message is strictly prohibited.  If you have received this communication in error, please notify us  immediately by reply email.  Thank you

Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you.
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
https://urldefense.com/v3/__http://lists.utsouthwestern.edu/mailman/listinfo/histonet__;!!JA_k2roV-A!FwKdOPOzj_UZTnMVxT9qbcCJsUPm3F4e3bDrGK4DAjDyfuxQPVCf46_156AghOFHjVm2UeSVHSgv4hZS98GaWcNtyAQS7nLY$ 

**********************************************************************
The information contained in this e-mail may be privileged, confidential, and/or protected from disclosure. If you are the intended recipient, further disclosures are prohibited without proper authorization. If you are not the intended recipient (or have received this e-mail in error) please notify the sender immediately and destroy this e-mail. Any unauthorized copying, disclosure or distribution of the material in this e-mail is strictly prohibited and no waiver of any attorney-client, work product, or other privilege is intended. No binding agreement on behalf of Baylor Scott & White Health, or any affiliated entity, is permitted by e-mail without express written confirmation by a duly authorized representative of Baylor Scott & White Health.