[Histonet] [External] unprocessed tissue and Excelsior AS issue

[Alonso Martínez Canabal]

Good morning,
        I want to perform immunohistochemistry in mouse postnatal brains of
0,5,10 days. I have seen that many people, including Paxinos, make the
sections including the skull. Do you think that I should follow this using
a protocol for decalcification of the skull?
Thank you!

El vie, 30 jun 2023 a las 13:21, Dessasau, Evan D. via Histonet (<
histonet at lists.utsouthwestern.edu>) escribió:

> Hi Mr. Horvath, I work at a Biomedical research facility with non-human
> primates as the main lab animal.  If you could use wet tissue from
> non-human primates, you could submit a Specimen Request for access to
> tissue for your project.  If interested, please email Mrs. Summerville
> cc'ed here.
> E-van
>
>
>
> -----Original Message-----
> From: Andrew Horvath via Histonet <histonet at lists.utsouthwestern.edu>
> Sent: Friday, June 30, 2023 2:41 PM
> To: histonet at lists.utsouthwestern.edu
> Subject: [External] [Histonet] unprocessed tissue and Excelsior AS issue
>
> Hello all,
>
>
>
> I have two things to address in this email.
>
>
>
> First, I am trying to locate tissue that has not been processed so we can
> use it for testing of our tissue processors.  Optimally it would be colon
> but also esophagus as we are a GI lab.  Does anyone know where I can find
> such tissue (in the US preferably)?
>
>
>
> Second, we use the Epredia Excelsior AS processor and have run into issues
> with the processing of tissue where portions of small biopsy specimens will
> have sections that appear to not be processed.  It does not happen on every
> specimen but is consistent enough we are trying to find solutions to this
> problem.  Hence the non-processed tissue I am trying to locate.
>
>
>
> Any help is appreciated!
>
>
>
>
>
>
>
> Thanks,
>
>
>
> Andrew
>
>
>
>
>
> Andrew Horvath, MBA, MA
>
> Business Operations Manager
>
> 303-220-4369
>
>
>
> Centennial Pathologists, PC
>
> 7346 South Alton Way, Suite 10-D
>
> Centennial, CO 80112
>
>
>
>
>
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-- 
Dr. Alonso Martínez Canabal PhD
Profesor Asociado "C"
Departamento de Biología Celular, Facultad de Ciencias, UNAM
Investigador Nacional "I"
56224833