[Histonet] ISH decal specimens

[Val Tolley]

Hi Kendra-

Decal Stat from Statlab is HCl based, therefore is not appropriate for subsequent IHC, ISH, or other molecular studies.  There is lots of peer-reviewed published data, if you need to supply rationale to your pathologists.  HCl is known to significantly reduce molecular assay sensitivity and cause false negatives.

Switch to either a formic-acid based decal (such as Immunocal) or EDTA-based decal and re-optimize your assay(s).

Val Tolley, HTL (ASCP), QIHC

> On Jun 23, 2023, at 8:53 AM, Kendra Beechie ND-Bismarck via Histonet <histonet at lists.utsouthwestern.edu> wrote:
> 
> Hello all,
> 
> Recently, the Kappa/Lambda ISH has quit working on our decal specimens.
> When we first validated ISH, we tested decal specimens that stained
> appropriately. We use the DECAL Stat from StatLab. I did NOT do the initial
> ISH validation back in 2019, but as far as I know, we have not changed
> which Decal solution we use since then.
> 
> Has anyone else experienced this problem? I realize the other option is to
> run K/L IHC on the decal specimens, but we discontinued those after we
> brought the ISH on board. My medical director wants me to try to isolate
> the problem. My troubleshooting has only revealed that the decal is the
> common denominator.
> 
> Thanks for any help!
> 
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