[Histonet] Peroxidase Stain

Terri Braud tbraud at holyredeemer.com
Tue Jun 13 13:46:14 CDT 2023 

I have long used the kits from Sigma and found them to be extremely reliable.  Call their tech helpline for advice, or reach out to your pathologist for their preference.  I know that "homemade" saves money over kits, but for me, my most costly and precious resource is my tech time.  Save yourself and your techs a headache and call Sigma-Aldrich.
My 2 cents, Terri

Terri L. Braud, HT(ASCP)
HNL Laboratories for 
Holy Redeemer Hospital
1648 Huntingdon Pike
Meadowbrook, PA 19046
Ph: 215-938-3689
Fax: 215-938-2021
          Honesty
AccouNtability
    AgiLity
    CoLlaboration
  CoMpassion

-----Original Message-----
From: histonet-request at lists.utsouthwestern.edu <histonet-request at lists.utsouthwestern.edu> 
Sent: Tuesday, June 13, 2023 1:00 PM
To: histonet at lists.utsouthwestern.edu
Subject: [EXTERNAL] Histonet Digest, Vol 235, Issue 9

CAUTION: This email originated from outside Redeemer Health. Do not click links or open attachments unless you recognize the sender and know the content is safe. Contact our IT Support Center at 215-938-3900 with questions.


Send Histonet mailing list submissions to
	histonet at lists.utsouthwestern.edu

To subscribe or unsubscribe via the World Wide Web, visit
	http://lists.utsouthwestern.edu/mailman/listinfo/histonet
or, via email, send a message with subject or body 'help' to
	histonet-request at lists.utsouthwestern.edu

You can reach the person managing the list at
	histonet-owner at lists.utsouthwestern.edu

When replying, please edit your Subject line so it is more specific than "Re: Contents of Histonet digest..."


Today's Topics:

   1. Peroxidase stain on peripheral smears (Kendra Beechie ND-Bismarck)
   2. MOHS staining (Ken M)
   3. Re: Peroxidase stain on peripheral smears (John Kiernan)


----------------------------------------------------------------------

Message: 1
Date: Mon, 12 Jun 2023 14:22:08 -0500
From: Kendra Beechie ND-Bismarck <kendra.beechie at commonspirit.org>
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Peroxidase stain on peripheral smears
Message-ID:
	<CADtF1soCXbTZ5dzAMw84L68dK0C4d4bQL-3-tYGaAgsQ9CKLaA at mail.gmail.com>
Content-Type: text/plain; charset="utf-8"

Hello,

I am seeking some guidance in regards to a peroxidase stain. We have always used the Kaplow Method to stain peripheral blood smears, and up until recently, we have had no issues with getting it to work.  However, no matter what we try in recent months, we have not been able to get it to work. Several different techs have tried it and we have ordered new reagents, but there are no granules to be seen....

Does anyone have any experience with peroxidase kits? I can see that Sigma-Aldrich has several available but I'm not sure what to go with....

Any and all help would be greatly appreciated!

Thank you,

Kendra Beechie
MLS/HTL (ASCP), Technical Consultant
CHI St. Alexius Health
Bismarck, ND

Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you.


------------------------------

Message: 2
Date: Mon, 12 Jun 2023 20:34:52 +0000
From: Ken M <kdean70 at hotmail.com>
To: "histonet at lists.utsouthwestern.edu"
	<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] MOHS staining
Message-ID:
	<MN2PR10MB31832CE000DBAED062E5A361AD54A at MN2PR10MB3183.namprd10.prod.outlook.com>
	
Content-Type: text/plain; charset="iso-8859-1"

For those doing MOHS staining either manually or automatically. How many staining cups do you really use? Are six enough if there is a running water cup nearby or do you need more?

Ken


------------------------------

Message: 3
Date: Tue, 13 Jun 2023 04:46:26 +0000
From: John Kiernan <jkiernan at uwo.ca>
To: "histonet at lists.utsouthwestern.edu"
	<histonet at lists.utsouthwestern.edu>,	Kendra Beechie ND-Bismarck
	<kendra.beechie at commonspirit.org>
Subject: Re: [Histonet] Peroxidase stain on peripheral smears
Message-ID:
	<YT2P288MB0404B7C498407EEE7DA4F7D4A555A at YT2P288MB0404.CANP288.PROD.OUTLOOK.COM>
	
Content-Type: text/plain; charset="iso-8859-1"

What is the Kaplow method? I can't find it in textbooks. A quick Google search brings up only junk papers indicating that a Kaplov method may use carcinogenic benzidine (with wrong spelling) as the chromogen.
There are simple, safe and inexpensive methods for histochemical localization of sites of peroxidase activity.in tissue sections or smears of cells. Buy a textbook for about $50, read it, and save your lab a fortune in buying special kits for very simple routine histochemical jobs.

My ten cents-worth. June 2023
John Kiernan
J. A. Kiernan MB, ChB, PhD, DSc
Professor Emeritus, Anatomy & Cell Biology University of Western Ontario, London, Canada https://www.schulich.uwo.ca/anatomy/people/bios/emeriti/kiernan_john.html
Also  Secretary, Biological Stain Commission, Inc.
https://biologicalstaincommission.org
= = =
________________________________
From: Kendra Beechie ND-Bismarck via Histonet <histonet at lists.utsouthwestern.edu>
Sent: June 12, 2023 3:22 PM
To: histonet at lists.utsouthwestern.edu <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Peroxidase stain on peripheral smears

Hello,

I am seeking some guidance in regards to a peroxidase stain. We have always used the Kaplow Method to stain peripheral blood smears, and up until recently, we have had no issues with getting it to work.  However, no matter what we try in recent months, we have not been able to get it to work. Several different techs have tried it and we have ordered new reagents, but there are no granules to be seen....

Does anyone have any experience with peroxidase kits? I can see that Sigma-Aldrich has several available but I'm not sure what to go with....

Any and all help would be greatly appreciated!

Thank you,

Kendra Beechie
MLS/HTL (ASCP), Technical Consultant
CHI St. Alexius Health
Bismarck, ND

Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you.
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

________________________________
From: Kendra Beechie ND-Bismarck via Histonet <histonet at lists.utsouthwestern.edu>
Sent: June 12, 2023 3:22 PM
To: histonet at lists.utsouthwestern.edu <histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Peroxidase stain on peripheral smears

Hello,

I am seeking some guidance in regards to a peroxidase stain. We have always used the Kaplow Method to stain peripheral blood smears, and up until recently, we have had no issues with getting it to work.  However, no matter what we try in recent months, we have not been able to get it to work. Several different techs have tried it and we have ordered new reagents, but there are no granules to be seen....

Does anyone have any experience with peroxidase kits? I can see that Sigma-Aldrich has several available but I'm not sure what to go with....

Any and all help would be greatly appreciated!

Thank you,

Kendra Beechie
MLS/HTL (ASCP), Technical Consultant
CHI St. Alexius Health
Bismarck, ND

Caution: This email is both proprietary and confidential, and not intended for transmission to (or receipt by) any unauthorized person(s). If you believe that you have received this email in error, do not read any attachments. Instead, kindly reply to the sender stating that you have received the message in error. Then destroy it and any attachments. Thank you.
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet


------------------------------

Subject: Digest Footer

_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

------------------------------

End of Histonet Digest, Vol 235, Issue 9
****************************************

More information about the Histonet mailing list