[Histonet] (no subject)

[Tony Reilly]

Hello Rhonda
I have extensive experience with the Brown Hopps stain.

I would advise against only staining for gram negative as both the gram positive stain and the gram negative stain will stain both gram positive and gram negative organisms.  The differentiation is only achieved by the destaining steps.

If you only used the gram negative steps you would still be staining both gram positive and gram negative organisms negatively.

If you you want more information let me know .

Regards
Tony Reilly
Histologist
Australia



Sent from my iPhone

> On 4 Jul 2023, at 5:30 am, Mac Donald, Jennifer via Histonet <histonet at lists.utsouthwestern.edu> wrote:
> We have good success with the Twort.
> 
> -----Original Message-----
> From: Rhonda McCormick via Histonet <histonet at lists.utsouthwestern.edu>
> Sent: Monday, July 3, 2023 11:22 AM
> To: Histonet <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] (no subject)
> 
>  EXTERNAL SENDER - Exercise caution with requests, links, and attachments.
> 
> Hello, I'm wondering if anyone does the Brown-Hopps Gram stain for gram negative bacteria. If so, would you mind to send your protocol please?
> Any recommendations for a gram negative (only) stain - specifically E. coli? One of our residents is asking.
> Thank you!
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