[Histonet] Possible causes for tissue tears

[Charles Riley]

Hello all,

I tried posting this question earlier but the file size was too big.

I processed a piece of tissue engineered cartilage the other day for a
research project and stained it with a few different stains. Each sample
has various tears through the tissue (that don't appear to be microtomy
artifacts in my opinion). I was hoping to get some feedback as to what
everyone thinks may be the most likely cause.   I have a image I can send
directly to you if you reply for added info.