[Histonet] shelf life of working antibody solutions in IHC

[Naira Margaryan]

Hi Tony,

Very interesting article you shared.

Hi Gudrun,

I will only add to this - run positive and negative tests for every run.

Good luck!
Naira

On Sat, Jul 1, 2023 at 4:40 PM Tony Henwood via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> Hi Gudrun,
>
> This should be useful ( I will send a copy separately)
>
> Henwood, A. F. (2023). Validation of nominally expired antibodies for
> immunohistochemistry. Biotechnic & Histochemistry, 98(2), 86-93.
>
> Regards,
>
> Tony Henwood MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA) (Retired)
> Principal Scientist, the Children’s Hospital at Westmead (Retired)
> Adjunct Fellow, School of Medicine, University of Western Sydney.
>
> From: Gudrun Lang via Histonet<mailto:histonet at lists.utsouthwestern.edu>
> Sent: Sunday, 2 July 2023 4:59 AM
> To: histonet at lists.utsouthwestern.edu<mailto:
> histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] shelf life of working antibody solutions in IHC
>
> Hi all!
>
> I have tried to find a general instruction for the shelf life of antibody
> working solutions.
>
> With automated IHC you usually fill the container with the working solution
> and depending on the frequency of usage they stay on the instrument at
> roomtemperature (or higher) or are put in the fridge again.
>
> The working solutions are up to 10 ml and may last for months. The
> antibody-diluent is from the same company of the instrument. The titers are
> in a range from 1:10 to 1:3000. - so a very heterogen situation.
>
>
>
> How do you handle this? Have you a general rule, when the solution has to
> be
> discarded? Is it just a matter of positiv-controls?
>
>
>
> Thanks in advance
>
> Gudrun Lang
>
> Biomedical scientist
>
> Austria
>
>
>
>
>
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