[Histonet] research questions

[Caroline Miller]

I would say it depends on the project. I would always strive to get
controls from the same species, but sometimes that is just not possible. If
the marker's cellular or tissue location is easily recognizable then Ok
with the different species, but if it was a diffuse and hard to interpret
marker then I would try harder to get an in-species control.

I don't think I would use a different species control to determine ideal
concentration / protocol for the main cohort. I would get it going on the
control and run the cohort and tweak from there with positive samples
(hopefully) in the cohort.

Length of fixation/processing/age of slides of the control vs cohort is
also something to think about.

yours,
Caroline



On Tue, Feb 21, 2023 at 12:53 PM Charles Riley via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> When performing IHC's for research projects is it recommended to use the
> same species for control tissues or will any tissues that the antibody has
> been validated for by the company be acceptable?
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-- 
Caroline Miller, M.Sc
Histology, Imaging, and Image Analysis Specialist
415 2187297
https://www.linkedin.com/in/mills42/