[Histonet] Mice brain

[John Kiernan]

Immersion in sucrose is for cryoprotection - to minimize damage from formation of ice crystals. It is needed only if you are cutting frozen sections. Specific demonstration of cholinergic neurons is with immunohistochemistry to detect choline acetyltransferase. Use frozen or paraffin sections, according to instructions that come with the primary antibody. For histochemical demonstration of acetylcholinesterase activity (seen also in some neurons that are not cholinergic), you need frozen sections of tissue fixed for 12h in neutral formaldehyde at 4oC. My favourite method for AChE activity was that of Karnovsky & Roots 1964 J. Histochem. Cytochem. 12:219-221.
John Kiernan (London, Canada).
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________________________________
From: Renee Fisher via Histonet <histonet at lists.utsouthwestern.edu>
Sent: April 19, 2023 1:34 PM
To: Histonet at lists.utsouthwestern.edu <Histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Mice brain

Hi,
Does anyone know if immersing in  sucrose is essential for optimum visualization of cholinergic neurons in mouse brain.

Thank you
Sent from my iPhone
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