[Histonet] Molds- cold vs warm

[Thomas Podawiltz]

Tell us how you really feel Jay. LOL. 
You say everything that I would say and have said while training people. 
Like you having been doing this for a bit (1980) and had  the privilege of meeting Mr. Lee Luna. 

 


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On Friday, September 9, 2022, 6:14 PM, Naira Margaryan via Histonet <histonet at lists.utsouthwestern.edu> wrote:

Thank You so much Jay, for such detailed explanation and for permission to
use your email to address.

My sincere regards,
Naira

On Fri, Sep 9, 2022 at 5:00 PM Cooper, Brian <bcooper at chla.usc.edu> wrote:

> Thanks for saying this Jay!! I have to say, it's been a while since we've
> had such a great response on Histonet!! Everything you said is spot on.
>
> Happy Friday everyone!
>
> Thanks,
>
> Brian Cooper
> Histology Supervisor
> Children's Hospital Los Angeles
> Sent from my mobile
>
>
>
>
>
> On Sep 9, 2022 2:37 PM, Jay Lundgren via Histonet <
> histonet at lists.utsouthwestern.edu> wrote:
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> This email came from outside CHLA. Do not open attachments, click on
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> address: histonet-bounces at lists.utsouthwestern.edu.
>
>
> Whoever is telling you to use cold molds needs to go back to clown college.
>
> That is totally, 100%, absolutely, wrong.
>
> There is some debate as to embed "wet" (cassettes submerged in paraffin
> bath) or "dry", and I will accept either, as mostly a matter of personal
> preference.  BUT, in both of these cases, the molds are hot.
>
> I have been a Histotech for five decades, trained at Armed Forces Institute
> of Pathology (back when that used to mean something) and I have NEVER seen
> anyone using cold molds.
>
> It is a guaranteed way to get cold fractures and cracks in your blocks, or
> to pop tissue out when you are cutting, which might be irretrievable. Just
> think how much time all those re-embedded blocks are going to save you!
>
> Also, you won't be able to easily re-position specimens in the block, to
> put them "on edge" or "on end", for example.  The tissue will instantly
> stick to the cold mold.  And if you want to re-position it, guess what,
> you'll have to warm the mold up to get the tissue unstuck.  How's that
> (non-existent anyway) time savings now?
>
>
> If you want to prove to whatever jackass suggested this that they are
> wrong, get a big stack of every histopathology textbook you can find.
> There is nothing in any of them talking about paraffin embedding with cold
> molds.
>
> As a matter of fact, every single textbook will specify molds at the same
> temp as paraffin.
>
> Anyway, it doesn't even make sense, thermodynamically.  Heat travels from
> hot to cold.  Those "cold" molds will be the same temperature as the
> paraffin, almost instantly. Did it take a tiny amount of heat out of the
> hot paraffin? Yes, but not enough to noticeably cool the blocks faster. The
> amount of heat from the paraffin used to warm the mold is trivial compared
> to the total heat of the system. That's why cold plates have huge, noisy
> refrigeration units.  You can't argue with thermodynamics.
>
> If you are having trouble getting your blocks to release, use mold
> release!  Viola!
> https://secure-web.cisco.com/1vhENcmRngDgLubdLEYMzzWWUK4ILg_WIJNnMutz67Oikk5LSg5SqF6OvSQqMWpr4MIirbF_ExGbIXm9Usdm35LUk87pXYTIvPVdKY5u2dRCdo_Ss-iuZ4nCOa0nPTIpPec8zwvOBcVIE7eM7o-flt9BAIGK0ZOw4K3HOXwNiLmQBnD0hFb9pgrU0ZuPnk5llOYCeJ5b2Pmkp2B9UPlVvxPMI3-iHRILtOB4kPL45PII_yUJnJhFYAryeid5lrITtm-w0KNyKrfJVI0mHy47Niz0TEpxxvl3DoTDmq-umsyN3BucCj2B-aJFqJ-AW3thtXSEk-Nl0NzBBrSxw8cPzSrKsVww7cCLh_krbh7VXKlRiRGF41o3UKk_oEQuHGIEeYlUNLnpLndnkSH0cwR3nNWhq3Cy8hw6ws0Ka8kYRH8_TVttsOh_lQbO4tm6_i-fdNOZxcR_7t-QeE9aW5YP1hg/https%3A%2F%2Fwww.statlab.com
> I used to think it was
> superfluous, but now I consider it compulsory.  This is probably the answer
> to most of your issues.
>
> I don't know who is suggesting using cold molds, but I can pretty much
> guarantee that it's a pathologist who thinks his slides are taking too
> long, and knows nothing about histopathology, or a lab manager, who knows
> nothing about histopathology.  This next part is directly to them.
>
> To Whoever Suggested Cold Molds:  The answer to getting your slides out
> quicker is buying more equipment and hiring more techs, and holding
> everyone to standards (30 blocks/hr cutting, 60 blocks/hr embedding).
> Making nonsensical, uninformed suggestions only exposes your ignorance.
>
> Please feel free to show them this reply.
>
> Sincerely,
>
> Jay A. Lundgren, M.S., HTL (ASCP)
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