[Histonet] Histonet Digest, Vol 225, Issue 14

Tue Sep 6 12:01:51 CDT 2022

Changing your IHC slides to another brand with stronger slide adhesion is
suggested when working with bone and very fatty tissue to remain on the
slide.  shaking off as much moisture from the slide rack before placing
them to dry in the oven is another.  Some animal tissues benefit from
leaving in the oven overnight at 60 degrees celsius.

regarding slides with better tissue section adhesion, our bone marrow
pathology laboratory likes using the Azer Scientific's EMS200W+ charged
slides. There's another brand that's great for human brain, breast and also
animal tissues: I believe they're called Autofrost by Cancer diagnostics
(but I'm not sure if it's these, as the slides were used mostly at another
laboratory I used to work at five years ago).  I hope this all helps!

Best regards,
Eddie Martin, HTL, QIHC
Bethesda, MD

On Wed, Aug 31, 2022 at 1:00 PM <histonet-request at lists.utsouthwestern.edu>
wrote:

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> Today's Topics:
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>    1. Automated ihc staining of bone (Charles Riley)
>    2. Re: Automated ihc staining of bone (Cooper, Brian)
>    3. Information on the resistance/viability of different
>       tissues/cells to different freezing temperatures? (Alida Bailleul)
>
>
>
> ---------- Forwarded message ----------
> From: Charles Riley <criley at udel.edu>
> To: histonet at lists.utsouthwestern.edu
> Cc:
> Bcc:
> Date: Tue, 30 Aug 2022 15:50:18 -0400
> Subject: [Histonet] Automated ihc staining of bone
> I am working on trying to get IHC stains optimized on bone samples of rat
> tibias. The issue I am running into is that the periosteum is
> separating from the bone marrow and wrinkling up over top of the rest of
> the sample.
>
> If anyone has any techniques to prevent this wrinkling/detachment of tissue
> from the main section during automated ihc staining it would be greatly
> appreciated.
>
>
>
>
> ---------- Forwarded message ----------
> From: "Cooper, Brian" <bcooper at chla.usc.edu>
> To: Charles Riley <criley at udel.edu>, "histonet at lists.utsouthwestern.edu" <
> histonet at lists.utsouthwestern.edu>
> Cc:
> Bcc:
> Date: Tue, 30 Aug 2022 20:09:14 +0000
> Subject: Re: [Histonet] Automated ihc staining of bone
> Charles,
>
> Try and blot your sections dry before placing them into the oven.  After
> picking up your section, place your slide onto a flat surface and take a
> paper towel or piece of filter paper, and gently press it down on top of
> the section to wick away all of the moisture.  We use this technique
> frequently here on bone marrow cores.
>
> Thanks,
>
> Brian D. Cooper, HT (ASCP)CM QIHC| Histology Supervisor
> Department of Pathology and Laboratory Medicine
> Children's Hospital Los Angeles
> 4650 Sunset Blvd MS#43- Los Angeles, CA 90027
> bcooper at chla.usc.edu
>
> -----Original Message-----
> From: Charles Riley via Histonet <histonet at lists.utsouthwestern.edu>
> Sent: Tuesday, August 30, 2022 12:50 PM
> To: histonet at lists.utsouthwestern.edu
> Subject: [Histonet] Automated ihc staining of bone (EXTERNAL EMAIL)
>
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> I am working on trying to get IHC stains optimized on bone samples of rat
> tibias. The issue I am running into is that the periosteum is separating
> from the bone marrow and wrinkling up over top of the rest of the sample.
>
> If anyone has any techniques to prevent this wrinkling/detachment of
> tissue from the main section during automated ihc staining it would be
> greatly appreciated.
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> ---------- Forwarded message ----------
> From: Alida Bailleul <alida.bailleul at gmail.com>
> To: histonet <histonet at lists.utsouthwestern.edu>
> Cc:
> Bcc:
> Date: Wed, 31 Aug 2022 10:27:56 +0800
> Subject: [Histonet] Information on the resistance/viability of different
> tissues/cells to different freezing temperatures?
> Where can I find information on the resistance of different tissues/cells
> (e.g.,  bone, cartilage) to different freezing temperatures (e.g., -4C;
> -24C; -70C ) ? I am asking about frozen tissues that were not
> cryopreserved. I think there may be a lot of experimental studies that
> compared the resistance of cells/tissues to freeze-thaw cycles at different
> temperatures (and also compared fresh frozen versus cryopreserved) but I
> didn't really find a lot of studies. Moreover studies don't really compare
> different tissue types, they focus on one type of tissue per study.
> Please guide me towards some papers (perhaps in the old literature? Even
> before cryopreservation was discovered?).
> I think there might be information in papers about how to store
> osteochondral grafts, but I did not find the information I was looking for.
> Thank you in advance
> Best wishes
>
> Alida
>
> --
> Dr. Alida M. Bailleul
> Associate Professor
> Institute of Vertebrate Paleontology and Paleoanthropology, Chinese Academy
> of Sciences
> www.ivpp-avianevolution.com
> & Research Associate of Paleontology, Museum of the Rockies, Montana State
> University
> Google Scholar
> <https://scholar.google.co.jp/citations?user=Yn4PuWMAAAAJ&hl=en> -
> ResearchGate
> <https://www.researchgate.net/profile/Alida_Bailleul>
>
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