[Histonet] Histonet Digest, Vol 226, Issue 1

[kidist Shamebo]

On Thu, Sep 1, 2022 at 12:00 PM <histonet-request at lists.utsouthwestern.edu>
wrote:

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>    1.  Automated ihc staining of bone (Bacon, Charles)
>
>
>
> ---------- Forwarded message ----------
> From: "Bacon, Charles" <Charles.Bacon at baystatehealth.org>
> To: "histonet at lists.utsouthwestern.edu" <histonet at lists.utsouthwestern.edu
> >
> Cc:
> Bcc:
> Date: Wed, 31 Aug 2022 17:23:34 +0000
> Subject: [Histonet]  Automated ihc staining of bone
> When we have section lifting during IHC, we will use a concentrated Poly-L
> Lysine solution. You can dilute this solution but instead we will use a
> direct application (wet the tip of a gloved finger with the solution, and
> wipe down the slide, let dry) on one of our charged slides. This may cause
> a degree of counter stain background but always improves the IHC result for
> the pathologist. We use this for bone or heavily keratinized skin sections.
>
> Supplies:
> Poly-L: Sigma Diagnostics™ Poly-L-lysine Solution - SDP8920A
> Slides: TYPENEX Medical Microscope Slides - PS0101P
>
> Chuck Bacon, HTL(ASCP)CM
> Supervisor Histology
> Baystate Medical Center
> 361 Whitney Ave., Holyoke, MA 01040
> Telephone: 413-322-4786  Fax: 413-322-4790
> Charles.Bacon at baystatehealth.org
>
> -----Original Message-----
> From: histonet-request at lists.utsouthwestern.edu <
> histonet-request at lists.utsouthwestern.edu>
> Sent: Wednesday, August 31, 2022 1:00 PM
> To: histonet at lists.utsouthwestern.edu
> Subject: Histonet Digest, Vol 225, Issue 14
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