[Histonet] cd138
Paula
plucas at biopath.org
Wed Oct 26 13:57:44 CDT 2022
Hello Histonetters,
We have a Bond III and use the Refine Detection Kit for our antibodies.
We've had no issues with this mentioned antibody but recently, we have seen
background staining and have used 2 different lots. Both the plasma cells
AND stromal cells are stained in endometrial biopsies.
The antibody is clone M115 and it is a predilute. The default protocol is
ER1 for 30 minutes. I have tried to lessen the time in ER1 but it comes out
the same.
Leica support suggests adding a couple of washes after the Polymer step.
Does anyone have any recommendations/thoughts?
Thank you so much in advance.
Paula
Laboratory Manager
Bio-Path Medical Group
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