[Histonet] IHC background

[人生过客$]

？？？


---原始邮件---


发件人：人生过客$ via Histonet"<histonet at lists.utsouthwestern.edu>


发送时间："undefined"


收件人："Tony Henwood (SCHN) via Histonet"<histonet at lists.utsouthwestern.edu>


主题：Re: [Histonet] IHC background

你理我不


---原始邮件---


发件人：Tony Henwood (SCHN) via Histonet"<histonet at lists.utsouthwestern.edu>


发送时间："undefined"


收件人："histonet at lists.utsouthwestern.edu"<histonet at lists.utsouthwestern.edu>


主题：Re: [Histonet] IHC background

Paula,

What does the artefact look like?

Another cause could be partial lifting of parts of the section, allowing reagents to become trapped under the section.
You commonly see it in thyroids where the colloid traps antibody and/or detection reagents under the colloid giving a particulate brown dust-like staining (sometimes looking like cocci).
You can also sometimes see it in fibrous tissue in sections.

Suggestions to solve this: check your sticky adhesive slides, or allow the slides to dry longer at room temperature before placing on the Bond (allows the adhesive time to work).

Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children’s Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA

This message is intended for the addressee named and may contain confidential information. If you are not the intended recipient, please delete it and notify the sender.

Views expressed in this message are those of the individual sender, and are not necessarily the views of NSW Health or any of its entities.
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet