[Histonet] IHC background

[Tony Henwood (SCHN)]

Paula,

What does the artefact look like?

Another cause could be partial lifting of parts of the section, allowing reagents to become trapped under the section.
You commonly see it in thyroids where the colloid traps antibody and/or detection reagents under the colloid giving a particulate brown dust-like staining (sometimes looking like cocci).
You can also sometimes see it in fibrous tissue in sections.

Suggestions to solve this: check your sticky adhesive slides, or allow the slides to dry longer at room temperature before placing on the Bond (allows the adhesive time to work).

Regards
Tony Henwood JP, MSc, BAppSc, GradDipSysAnalys, CT(ASC), FFSc(RCPA)
Principal Scientist, the Children’s Hospital at Westmead
Adjunct Fellow, School of Medicine, University of Western Sydney
Tel: 612 9845 3306
Fax: 612 9845 3318
Pathology Department
the children's hospital at westmead
Cnr Hawkesbury Road and Hainsworth Street, Westmead
Locked Bag 4001, Westmead NSW 2145, AUSTRALIA

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