[Histonet] roll plate - using freezing spray in a cryostat

Terri Braud tbraud at holyredeemer.com
Wed May 25 12:46:37 CDT 2022 


Terri L. Braud, HT(ASCP)
HNL Laboratories for 
Holy Redeemer Hospital
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Meadowbrook, PA 19046
Ph: 215-938-3689
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Today's Topics:

   1. Cryostat anti-roll plate (Ken Marzinsky)
   2. Re: Cryostat anti-roll plate (Keyser, Geri L)
   3. Re: Cryostat anti-roll plate (Anne Murvosh)
   4. Re: Cryostat anti-roll plate (Keyser, Geri L)
   5. Re: Cryostat anti-roll plate (Keyser, Geri L)


----------------------------------------------------------------------

Message: 1
Date: Tue, 24 May 2022 20:16:10 +0000
From: Ken Marzinsky <kdean70 at hotmail.com>
To: "histonet at lists.utsouthwestern.edu"
	<histonet at lists.utsouthwestern.edu>
Subject: [Histonet] Cryostat anti-roll plate
Message-ID:
	<BYAPR03MB4822A0499148ED2983D69E05ADD79 at BYAPR03MB4822.namprd03.prod.outlook.com>
	
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Hi everyone. Does anyone regularly use the anti-roll plate on their cryostat? Does it work well on the Leicas and Microms, or do you have to figit with it a lot? Does anyone have any tricks they can share to get it to work all the time?  I found that a little spritz of freeze spray on the plate and the bottom of the roll glass helps, but it is still seems a lot easier for us to use a brush or a teasing needle.

Ken
Durham, NC


------------------------------

Message: 2
Date: Wed, 25 May 2022 12:15:06 +0000
From: "Keyser, Geri L" <GKeyser at uwhealth.org>
To: "histonet at lists.utsouthwestern.edu"
	<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Cryostat anti-roll plate
Message-ID:
	<DM6PR17MB410180B500CF5D01BF99A8AAC4D69 at DM6PR17MB4101.namprd17.prod.outlook.com>
	
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I use roll plate. 

I don't like the roll plate on the Leica. The Leica design creeps... a lot. 

Also, with the leica, the contact point with the blade is a soft aluminum arm. If you make adjustments with the roll plate holder contacting the blade, it will damage the soft aluminum holder. 

I like the thermo design much better. It creeps a lot less. 

Here's a procedure for roll plate adjustment. 

1.	Mount block on specimen disc. 
2.	Place Specimen Disc in chuck, position Blade carrier, and face off.
3.	Turn knurling screw clockwise until sections do not pass under the Anti-roll plate.
4.	Turn knurling screw counter-clockwise by 20 degree increments until sections effortlessly pass under the Anti-Roll Plate WITHOUT hitting the block. 

*	If the block is hitting the anti-roll plate, it may cause damage to glass. 
*	Hard contact is visible and can be heard. 
*	Soft contact, which can also damage the Anti-roll plate, cannot be seen or heard. But, can be felt by gently placing finger on the anti-roll plate turning lever while taking a section.


Geri 


-----Original Message-----
From: Ken Marzinsky via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Tuesday, May 24, 2022 3:16 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Cryostat anti-roll plate

WARNING: This email appears to have originated outside of the UW Health email system.
DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe.

Hi everyone. Does anyone regularly use the anti-roll plate on their cryostat? Does it work well on the Leicas and Microms, or do you have to figit with it a lot? Does anyone have any tricks they can share to get it to work all the time?  I found that a little spritz of freeze spray on the plate and the bottom of the roll glass helps, but it is still seems a lot easier for us to use a brush or a teasing needle.

Ken
Durham, NC
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------------------------------

Message: 3
Date: Wed, 25 May 2022 13:32:12 +0000
From: Anne Murvosh <amurvosh at advancederm.net>
To: Ken Marzinsky <kdean70 at hotmail.com>,
	"histonet at lists.utsouthwestern.edu"
	<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Cryostat anti-roll plate
Message-ID:
	< 
st them. They creep forward so much that I just automatically know to roll iMW4PR15MB45615D24E17F493A42281080C6D69 at MW4PR15MB4561.namprd15.prod.outlook.com>
	
Content-Type: text/plain; charset="us-ascii"

We have 3 new leicas and we constantly have to adju t slightly back after every 4 specimens or so. The new leica designs are just bad and customer service is even worse. We have to use the plate as we do Mohs specimens and the sections are just better with the glass plate. I want to look for a different machine next time. We did test the Avantik and it cut nice but the glass plate was on the front and got in the way. I wish they would talk to Mohs techs before they design these. Oh well. No tricks just constant adjusting. Thanks Anne

-----Original Message-----
From: Ken Marzinsky via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Tuesday, May 24, 2022 1:16 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Cryostat anti-roll plate

Hi everyone. Does anyone regularly use the anti-roll plate on their cryostat? Does it work well on the Leicas and Microms, or do you have to figit with it a lot? Does anyone have any tricks they can share to get it to work all the time?  I found that a little spritz of freeze spray on the plate and the bottom of the roll glass helps, but it is still seems a lot easier for us to use a brush or a teasing needle.

Ken
Durham, NC
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HIPAA Confidentiality Notice: The information and documents accompanying this e-mail may contain confidential information that is legally privileged and protected by federal and state law. The information is intended for use only by the entity or individual to whom it is addressed, the authorized recipient. The authorized recipient is obligated to maintain the information in a safe, secure, and confidential manner. The authorized recipient is prohibited from using the information for purposes other than intended, prohibited from disclosing the information to any other party unless required to do so by law or regulation, and is required to destroy the information after its stated need has been fulfilled. If you are in possession of this information, and are not the intended recipient, you are hereby notified that any improper disclosure, copying, or distribution of the information is strictly prohibited. Please notify the owner of the information immediately and arrange for its return or destruction.



------------------------------

Message: 4
Date: Wed, 25 May 2022 14:59:04 +0000
From: "Keyser, Geri L" <GKeyser at uwhealth.org>
To: Anne Murvosh <amurvosh at advancederm.net>, Ken Marzinsky
	<kdean70 at hotmail.com>, "histonet at lists.utsouthwestern.edu"
	<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Cryostat anti-roll plate
Message-ID:
	<DM6PR17MB410181F7C2A9DCD736E68FD9C4D69 at DM6PR17MB4101.namprd17.prod.outlook.com>
	
Content-Type: text/plain; charset="us-ascii"

Maybe there's a product that increases friction with screw threads, doesn't have toxic outgassing, and works at low temperatures. 

I'm going to look for something like this. 

Geri

-----Original Message-----
From: Anne Murvosh via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Wednesday, May 25, 2022 8:32 AM
To: Ken Marzinsky <kdean70 at hotmail.com>; histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Cryostat anti-roll plate

WARNING: This email appears to have originated outside of the UW Health email system.
DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe.

We have 3 new leicas and we constantly have to adjust them. They creep forward so much that I just automatically know to roll it slightly back after every 4 specimens or so. The new leica designs are just bad and customer service is even worse. We have to use the plate as we do Mohs specimens and the sections are just better with the glass plate. I want to look for a different machine next time. We did test the Avantik and it cut nice but the glass plate was on the front and got in the way. I wish they would talk to Mohs techs before they design these. Oh well. No tricks just constant adjusting. Thanks Anne

-----Original Message-----
From: Ken Marzinsky via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Tuesday, May 24, 2022 1:16 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Cryostat anti-roll plate

Hi everyone. Does anyone regularly use the anti-roll plate on their cryostat? Does it work well on the Leicas and Microms, or do you have to figit with it a lot? Does anyone have any tricks they can share to get it to work all the time?  I found that a little spritz of freeze spray on the plate and the bottom of the roll glass helps, but it is still seems a lot easier for us to use a brush or a teasing needle.

Ken
Durham, NC
_______________________________________________
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Histonet at lists.utsouthwestern.edu
https://nam11.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C01%7Camurvosh%40advancederm.net%7Cd00ec7fb978f48d5784b08da3dc25292%7Cc026b5a4e9b54bdfb53b7130122cdb95%7C0%7C0%7C637890202080603264%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=Uhz9rhvCBTV95t%2BNEs0ocizQBXV7aAQyxswaE2Thm2E%3D&reserved=0
HIPAA Confidentiality Notice: The information and documents accompanying this e-mail may contain confidential information that is legally privileged and protected by federal and state law. The information is intended for use only by the entity or individual to whom it is addressed, the authorized recipient. The authorized recipient is obligated to maintain the information in a safe, secure, and confidential manner. The authorized recipient is prohibited from using the information for purposes other than intended, prohibited from disclosing the information to any other party unless required to do so by law or regulation, and is required to destroy the information after its stated need has been fulfilled. If you are in possession of this information, and are not the intended recipient, you are hereby notified that any improper disclosure, copying, or distribution of the information is strictly prohibited. Please notify the owner of the information immediately and arrange for its return or destruction.

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------------------------------

Message: 5
Date: Wed, 25 May 2022 15:02:25 +0000
From: "Keyser, Geri L" <GKeyser at uwhealth.org>
To: "Keyser, Geri L" <GKeyser at uwhealth.org>, Anne Murvosh
	<amurvosh at advancederm.net>, Ken Marzinsky <kdean70 at hotmail.com>,
	"histonet at lists.utsouthwestern.edu"
	<histonet at lists.utsouthwestern.edu>
Subject: Re: [Histonet] Cryostat anti-roll plate
Message-ID:
	<DM6PR17MB41017AB0B76F4A1EC82AB2DDC4D69 at DM6PR17MB4101.namprd17.prod.outlook.com>
	
Content-Type: text/plain; charset="utf-8"

Got it...

I need to test it. 

But, I'm going to take some parafilm, pull it over the bolt, then replace the screw. 

Increase that friction. ?

Geri 

-----Original Message-----
From: Keyser, Geri L via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Wednesday, May 25, 2022 9:59 AM
To: Anne Murvosh <amurvosh at advancederm.net>; Ken Marzinsky <kdean70 at hotmail.com>; histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Cryostat anti-roll plate

Maybe there's a product that increases friction with screw threads, doesn't have toxic outgassing, and works at low temperatures. 

I'm going to look for something like this. 

Geri

-----Original Message-----
From: Anne Murvosh via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Wednesday, May 25, 2022 8:32 AM
To: Ken Marzinsky <kdean70 at hotmail.com>; histonet at lists.utsouthwestern.edu
Subject: Re: [Histonet] Cryostat anti-roll plate

WARNING: This email appears to have originated outside of the UW Health email system.
DO NOT CLICK on links or attachments unless you recognize the sender and know the content is safe.

We have 3 new leicas and we constantly have to adjust them. They creep forward so much that I just automatically know to roll it slightly back after every 4 specimens or so. The new leica designs are just bad and customer service is even worse. We have to use the plate as we do Mohs specimens and the sections are just better with the glass plate. I want to look for a different machine next time. We did test the Avantik and it cut nice but the glass plate was on the front and got in the way. I wish they would talk to Mohs techs before they design these. Oh well. No tricks just constant adjusting. Thanks Anne

-----Original Message-----
From: Ken Marzinsky via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Tuesday, May 24, 2022 1:16 PM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Cryostat anti-roll plate

Hi everyone. Does anyone regularly use the anti-roll plate on their cryostat? Does it work well on the Leicas and Microms, or do you have to figit with it a lot? Does anyone have any tricks they can share to get it to work all the time?  I found that a little spritz of freeze spray on the plate and the bottom of the roll glass helps, but it is still seems a lot easier for us to use a brush or a teasing needle.

Ken
Durham, NC
_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
https://nam11.safelinks.protection.outlook.com/?url=http%3A%2F%2Flists.utsouthwestern.edu%2Fmailman%2Flistinfo%2Fhistonet&data=05%7C01%7Camurvosh%40advancederm.net%7Cd00ec7fb978f48d5784b08da3dc25292%7Cc026b5a4e9b54bdfb53b7130122cdb95%7C0%7C0%7C637890202080603264%7CUnknown%7CTWFpbGZsb3d8eyJWIjoiMC4wLjAwMDAiLCJQIjoiV2luMzIiLCJBTiI6Ik1haWwiLCJXVCI6Mn0%3D%7C3000%7C%7C%7C&sdata=Uhz9rhvCBTV95t%2BNEs0ocizQBXV7aAQyxswaE2Thm2E%3D&reserved=0
HIPAA Confidentiality Notice: The information and documents accompanying this e-mail may contain confidential information that is legally privileged and protected by federal and state law. The information is intended for use only by the entity or individual to whom it is addressed, the authorized recipient. The authorized recipient is obligated to maintain the information in a safe, secure, and confidential manner. The authorized recipient is prohibited from using the information for purposes other than intended, prohibited from disclosing the information to any other party unless required to do so by law or regulation, and is required to destroy the information after its stated need has been fulfilled. If you are in possession of this information, and are not the intended recipient, you are hereby notified that any improper disclosure, copying, or distribution of the information is strictly prohibited. Please notify the owner of the information immediately and arrange for its return or destruction.

_______________________________________________
Histonet mailing list
Histonet at lists.utsouthwestern.edu
http://lists.utsouthwestern.edu/mailman/listinfo/histonet

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------------------------------

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