[Histonet] fresh frozen OCT samples, high quality RNA extraction

M.O. modz9636 at gmail.com
Thu Feb 3 11:43:05 CST 2022


Hello,

This might be a longshot, but has anyone had success in extracting high
quality RNA from fresh frozen OCT samples/sections?

I'm trying to get high RINs from human samples and succeeded only once (out
of what seems to be 1 million attempts). I've trimmed off as much OCT as
possible, and even picked the sample out of the OCT when sectioning.

Any tips or hints would be most appreciated.

Sincerely,
Merissa


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