[Histonet] Histonet Digest, Vol 228, Issue 13

[John O’Brien]

Hi Chris
My company has large selection of refurbished and slightly  used Leica cryostats all models,  clinical and research
Please contact me for complete list and details ,all instruments come complete with full warranty 
Regards
John
IMEB Inc
www.imebinc.com

> On Nov 23, 2022, at 10:11 AM, histonet-request at lists.utsouthwestern.edu wrote:
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> Today's Topics:
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>   1. Cryostat Shopping (Chris England)
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> Message: 1
> Date: Wed, 23 Nov 2022 01:34:41 +0000
> From: Chris England <JoeAmateur at hotmail.com>
> To: "histonet at lists.utsouthwestern.edu"
>    <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Cryostat Shopping
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> Aloha Histonetters--I've missed you!
> 
>  1.  It's been a very long time since I last posted here so please forgive my heap of ignorance: I haven't done this in a hot minute.
>  2.  I think I'm in the market for a cryostat! But it's been 20+ years since the last one I bought, and I have no clue what features I'll need now. Do I buy a reliable 90s analog model? Do I need digital control? No idea! I'd love to talk to sales reps to get the pitch...but because I'm most likely to buy a used model, I feel like I'd be wasting their time. So I thought I'd ask you fine people.
> 
> TL;DR: What features would you recommend are very useful for a cryostat for a low-volume research lab? And is it a bad idea to expect to do H&E and other stains on cryosectioned tissue?
> 
> To get the best advice, I'll try to anticipate some of the more likely questions and answer:
> "What do you want to do?"
> Ideally, we'd like to do most of the same staining that one would do on FFPE tissue (H&E and some other stains) on fixed or unfixed frozen tissue. However more importantly I anticipate a lot of IHC/IF, and probably most important to us is preserving lipids in sectioned tissue (hence avoiding the clearing agents used with paraffin).
> "What kind of tissue?" We're a tissue engineering company working extensively with mammary cells, so the workload will likely be fatty tissue, and some composite samples that are tissue and polymer scaffolds (nothing a good blade can't easily cut--I think).
> "What volume?" Pretty low. I may well be underestimating the workload, but most likely a dozen blocks every few weeks.
> "What budget?" That what I need help with. I can probably go up to $20k, and mgmt is OK with a new model if it's necessary, but frankly I wasn't fantastic on a cryostat the last time I bought one so I'm not confident I'm qualified to say what exactly we need.
> "What is your skill level?" Noob. I've used a cryostat before, but my technique...definitely needs improvement. On the other hand, I'm the only one in my company that has any experience with histology at all, so I anticipate training a new generation of research histologists. So, I probably want a cryostat that may not do everything for me, but also won't require a lot of shoehorning.
> 
> I'll also leave a more open question down here: What is a paraffin microtome better suited to than a cryostat, and vice-versa?
> 
> Thank you so, so much in advance for your time and any help you can offer!!!
> 
> Warmest regards and aloha,
> Jack England
> 
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> End of Histonet Digest, Vol 228, Issue 13
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