[Histonet] Automated ihc staining of bone

[Bacon, Charles]

When we have section lifting during IHC, we will use a concentrated Poly-L Lysine solution. You can dilute this solution but instead we will use a direct application (wet the tip of a gloved finger with the solution, and wipe down the slide, let dry) on one of our charged slides. This may cause a degree of counter stain background but always improves the IHC result for the pathologist. We use this for bone or heavily keratinized skin sections. 

Supplies:
Poly-L: Sigma Diagnostics™ Poly-L-lysine Solution - SDP8920A
Slides: TYPENEX Medical Microscope Slides - PS0101P

Chuck Bacon, HTL(ASCP)CM 
Supervisor Histology
Baystate Medical Center
361 Whitney Ave., Holyoke, MA 01040
Telephone: 413-322-4786  Fax: 413-322-4790
Charles.Bacon at baystatehealth.org

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Sent: Wednesday, August 31, 2022 1:00 PM
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Subject: Histonet Digest, Vol 225, Issue 14

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