[Histonet] Immunoflouresence staining

Colleen Forster cforster at umn.edu
Wed Aug 24 14:16:49 CDT 2022


In my experience if the antibody are designed for flow they rarely work on
tissue samples.

Colleen Forster HT(ASCP)QIHC
University of MN

On Wed, Aug 24, 2022 at 1:56 PM Charles Riley via Histonet <
histonet at lists.utsouthwestern.edu> wrote:

> I have been given an antibody that is used for flow cytometry yet the
> researcher wants to use it for IF staining.  The protocol they provided is
> for the flow cytometry staining. Will this produce the immunoflourescense
> results they are looking for or do i need to use a different method?
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-- 
Colleen Forster HT(ASCP)QIHC
BLS Histology and IHC Laboratory
Jackson Hall, Room 2-155
321 Church St. SE
Minneapolis, MN 55455
612-626-1930


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