[Histonet] [External] Clarification about Immunohistochemistry

[Bitting, Angela K.]

We use an Azure B counterstain. Melanin has a greenish tinge after counterstaining.

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From: jayalakshmy p.s via Histonet <histonet at lists.utsouthwestern.edu> 
Sent: Monday, April 11, 2022 11:59 PM
To: histonet at lists.utsouthwestern.edu
Subject: [External] [Histonet] Clarification about Immunohistochemistry

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Hai all Histonetters
 Please anybody clarify my this doubt if possible.
When doing Immunohistochemistry for confirmation of Melanoma with DAB chromogen(brown color)the interpretation is not possible because of obscuring by the dense melanin pigment. We dont have any other color chromogen. I tried ihc after bleaching but the section gets detached even from charged slides. Is there any other effective way to do this?
Thanks in advance
Regards
Dr. P S Jayalakshmy
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