[Histonet] Clarification about Immunohistochemistry

jayalakshmy p.s psjayalakshmy at gmail.com
Mon Apr 11 22:58:42 CDT 2022


Hai all Histonetters
 Please anybody clarify my this doubt if possible.
When doing Immunohistochemistry for confirmation of Melanoma with DAB
chromogen(brown color)the interpretation is not possible because of
obscuring by the dense melanin pigment. We dont have any other color
chromogen. I tried ihc after bleaching but the section gets detached even
from charged slides. Is there any other effective way to do this?
Thanks in advance
Regards
Dr. P S Jayalakshmy


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