[Histonet] Question for Research on Bone

[Porter, Amy]

So I am working with Canine femurs and tibias that have been dissected as medial and lateral - they have been decaled in formic acid - rinsed in running tap well - processed on a routine 1 hour processing program ..... cutting very well!!

My issue is that the articular cartilage on the bone surface keep flipping up and over onto itself - I am using a 37C slide warmer overnight to allow them to hopefully flatten out more than on the water bath and then drying in the 56C oven for about 1 hour - then staining on automated Leica stainer - they are intact at 37..... look like the might be curling at 56C??

Anyone have any suggestions???

TIA - Amy

Amy S. Porter, HT (ASCP) - Lab Supervisor
Michigan State University
Investigative HistoPathology Lab
567 Wilson Road - Rm 2133
East Lansing, MI  48824
Ph:  517-884-5026
(she/her/hers)
https://sites.google.com/msu.edu/ihpl/home<https://urldefense.com/v3/__https:/sites.google.com/msu.edu/ihpl/home__;!!HXCxUKc!gaNWP-BGXxBxNF3HkuINL65j6K4xY5fN6q1y0Pb9w0qNLYZjcV7-CHy_BQT42A$>
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