[Histonet] Stain line order

Mac Donald, Jennifer jmacdonald at mtsac.edu
Tue Oct 26 11:58:44 CDT 2021

That step is known as differentiation.  Clearing involves an organic solvent to prepare the slides for coverslipping in a synthetic mounting medium.  
A regressive H&E uses differentiation, usually with 1% acid alcohol.  A progressive stain does not.  Some companies use a modified progressive using clarifier which is an acid alcohol using acetic acid.

-----Original Message-----
From: Anne Murvosh <amurvosh at advancederm.net> 
Sent: Tuesday, October 26, 2021 9:56 AM
To: Mac Donald, Jennifer <jmacdonald at mtsac.edu>
Subject: RE: Stain line order

  EXTERNAL SENDER- Exercise caution with requests, links, and attachments.

Clear; the tap water makes a blue film on the slides. I think this person used to use HCL water. I used clarifier 2 in the past.  Thanks Anne

-----Original Message-----
From: Mac Donald, Jennifer <jmacdonald at mtsac.edu>
Sent: Tuesday, October 26, 2021 9:49 AM
To: Anne Murvosh <amurvosh at advancederm.net>
Subject: RE: Stain line order

Clear or differentiate?

-----Original Message-----
From: Anne Murvosh via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Tuesday, October 26, 2021 9:27 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Stain line order

  EXTERNAL SENDER- Exercise caution with requests, links, and attachments.

When doing frozen section staining for Mohs we have DI water and don't need a clearing agent. I am helping set up someone who is using tap water and needs to clear it. They mentioned that they used to clear first, rinse, then use bluing. I thought it was the opposite after heme. Rinse, bluing, rinse, clearing. I don't remember which is first. Actually do you need a bluing if using tap water since that blues better then DI water? Thanks for your input, it's been 10 years since I had to think about this. Anne

Anne Murvosh
Histology Technician

This electronic transmission and any documents accompanying this electronic transmission may contain information that is confidential and/or legally privileged. The information is intended only for the use of the individual or entity named above. If you are not the intended recipient, you are hereby notified that any disclosure, copying, distribution or the taking of any action in reliance on or regarding the contents of this electronically transmitted information is strictly prohibited. If you have received this e-mail in error, please notify the sender and delete this message immediately.

HIPAA Confidentiality Notice: The information and documents accompanying this e-mail may contain confidential information that is legally privileged and protected by federal and state law. The information is intended for use only by the entity or individual to whom it is addressed, the authorized recipient. The authorized recipient is obligated to maintain the information in a safe, secure, and confidential manner. The authorized recipient is prohibited from using the information for purposes other than intended, prohibited from disclosing the information to any other party unless required to do so by law or regulation, and is required to destroy the information after its stated need has been fulfilled. If you are in possession of this information, and are not the intended recipient, you are hereby notified that any improper disclosure, copying, or distribution of the information is strictly prohibited. Please notify the owner of the information immediately and arrange for its return or destruction.
Histonet mailing list
Histonet at lists.utsouthwestern.edu

More information about the Histonet mailing list