[Histonet] Stain line order

[Mac Donald, Jennifer]

That step is known as differentiation.  Clearing involves an organic solvent to prepare the slides for coverslipping in a synthetic mounting medium.  
A regressive H&E uses differentiation, usually with 1% acid alcohol.  A progressive stain does not.  Some companies use a modified progressive using clarifier which is an acid alcohol using acetic acid.

-----Original Message-----
From: Anne Murvosh <amurvosh at advancederm.net> 
Sent: Tuesday, October 26, 2021 9:56 AM
To: Mac Donald, Jennifer <jmacdonald at mtsac.edu>
Subject: RE: Stain line order

  EXTERNAL SENDER- Exercise caution with requests, links, and attachments.

Clear; the tap water makes a blue film on the slides. I think this person used to use HCL water. I used clarifier 2 in the past.  Thanks Anne

-----Original Message-----
From: Mac Donald, Jennifer <jmacdonald at mtsac.edu>
Sent: Tuesday, October 26, 2021 9:49 AM
To: Anne Murvosh <amurvosh at advancederm.net>
Subject: RE: Stain line order

Clear or differentiate?

-----Original Message-----
From: Anne Murvosh via Histonet <histonet at lists.utsouthwestern.edu>
Sent: Tuesday, October 26, 2021 9:27 AM
To: histonet at lists.utsouthwestern.edu
Subject: [Histonet] Stain line order

  EXTERNAL SENDER- Exercise caution with requests, links, and attachments.

When doing frozen section staining for Mohs we have DI water and don't need a clearing agent. I am helping set up someone who is using tap water and needs to clear it. They mentioned that they used to clear first, rinse, then use bluing. I thought it was the opposite after heme. Rinse, bluing, rinse, clearing. I don't remember which is first. Actually do you need a bluing if using tap water since that blues better then DI water? Thanks for your input, it's been 10 years since I had to think about this. Anne


Anne Murvosh
Histology Technician



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