[Histonet] Dry Film Coverslipping?
Rene J Buesa
rjbuesa at yahoo.com
Thu Oct 7 07:54:19 CDT 2021
A Sakura film cover slipper will not work without xylene.Now that you have decided to go "xylene free" just go "all the way in" and after staining the slides DRY them in an oven at 60ºC during 5 minutes and cover.If you want further information on "cover slipping without xylene" send me your e-mail address and I will send you a copy of a short paper I published on this subject.René
On Wednesday, October 6, 2021, 01:17:48 PM GMT-4, Cates, Julia via Histonet <histonet at lists.utsouthwestern.edu> wrote:
I have been asked to consider going Xylene free. The only hang up that I am having is the coverslipping part. We have an old Sakura Film coverslipper and I don't know how that would work without xylene. When I did some searching in the archives, I found some comments made by Rene, that he used this type of coverslipper on oven dried slides but never referenced how. Does anyone use this method in your lab? If so, would you share your protocol and are there any problems to consider?
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