[Histonet] Histonet Digest, Vol 216, Issue 5

[Linda Hines]

STOP

On Sat, Nov 13, 2021, 11:00 AM <histonet-request at lists.utsouthwestern.edu>
wrote:

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> Today's Topics:
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>    1. Re: Paraffin embedding following storage in 70% alcohol
>       (John Kiernan)
>
>
>
> ---------- Forwarded message ----------
> From: John Kiernan <jkiernan at uwo.ca>
> To: "histonet at lists.utsouthwestern.edu" <histonet at lists.utsouthwestern.edu>,
> Charles Riley <criley at udel.edu>
> Cc:
> Bcc:
> Date: Fri, 12 Nov 2021 22:42:06 +0000
> Subject: Re: [Histonet] Paraffin embedding following storage in 70% alcohol
> Of course you are right!
> This is yet another example of an error in a procedure informally handed
> on from person to person! Always work from a book. Even a very old one will
> be OK for paraffin embedding.
> John Kiernan.
>
> https://www.schulich.uwo.ca/anatomy/people/faculty/emeriti/kiernan_john.html
> = = =
> ________________________________
> From: Charles Riley via Histonet <histonet at lists.utsouthwestern.edu>
> Sent: November 12, 2021 11:03 AM
> To: histonet at lists.utsouthwestern.edu <histonet at lists.utsouthwestern.edu>
> Subject: [Histonet] Paraffin embedding following storage in 70% alcohol
>
> I am working with a grad student on a project dealing with equine articular
> cartilage.
>
> The protocol she sent me for embedding the tissue samples goes directly
> from 70% alcohol to the embedding step in paraffin.
>
>
> Correct me if I am wrong but shouldn't the tissue be dehydrated fully and
> cleared before embedding the samples?
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