[Histonet] Paraffin embedding following storage in 70% alcohol

Hobbs, Carl carl.hobbs at kcl.ac.uk
Sat Nov 13 12:53:40 CST 2021


The Most venerable Histologist ( JK) is correct...as always!
No way to go from 70% alcohol directly into Pwax
Have to remove ALL water before infiltrating with Pwax
Yes....also need to replace alcohol with Xylene/Histoclear before infil. with Pwax.
Sure, you can use Isopropyl alc to obviate xylene /Histoclear/equiv. but......Jury's out on that
Carl Hobbs FIBMS
Histology and Imaging Manager
Wolfson CARD
Guys Campus, London Bridge 
Kings College London
London
SE1 1UL
 


020 7848 6810


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