[Histonet] IF with permanent mounting media?

[Paula Keene Pierce]

Hi,
you can use "Crystal Mount" first, let it dry, and then coverslip with xylene/resin.
I do this routinely with AEC IHC and oil red O and other fat stains.
Crystal Mount only on retinal flat mounts to be shipped out to others for future staining, as it can be removed with water,

FYI: Crystal mount is no longer made under that name, but do a search on Electron Microscopy Sciences website and their version will come up in the results.
Paula Keene Pierce, BS, HTL(ASCP)HTPresidentExcalibur Pathology, Inc.5830 N Blue Lake DriveNorman, OK 73069PH 405-759-3953http://www.excaliburpathology.com

A sharp knife is nothing without a sharp eye. - Klingon Proverb 

    On Friday, May 28, 2021, 10:43:39 AM CDT, Morken, Timothy via Histonet <histonet at lists.utsouthwestern.edu> wrote:  
 
 Has anyone tried using xylene/permanent mounting media for immunofluorescence stains? I had a question from a pathologist who wondered if we could do this. I have never heard of anyone doing it.

Tim Morken
Supervisor, Electron Microscopy/Neuromuscular Special Studies
Department of Pathology
UC San Francisco Medical Center

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